S(A) GENE AND BLOOD-PRESSURE COSEGREGATION USING DAHL SALT-SENSITIVE RATS

被引：73

作者：

HARRIS, EL ^{[1
]}

DENE, H ^{[1
]}

RAPP, JP ^{[1
]}

机构：

[1] MED COLL OHIO,DEPT MED,POB 10008,TOLEDO,OH 43699

来源：

AMERICAN JOURNAL OF HYPERTENSION | 1993年 / 6卷 / 04期

基金：

美国国家卫生研究院;

关键词：

GENETIC HYPERTENSION; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; ALTERNATE MESSENGER RNA SPLICING; DAHL SALT-SENSITIVE RAT;

D O I：

10.1093/ajh/6.4.330

中图分类号：

R6 [外科学];

学科分类号：

1002 ; 100210 ;

摘要：

Based on the published cDNA base sequence for the (anonymous) S(A) gene, a polymerase-chain-reaction (PCR) product from the S(A) gene was obtained from a Dah1 salt-sensitive rat kidney cDNA library. Compared to the published sequence, this product had a 102 base pair insert in the 3' end of the cDNA, probably as the result of alternate mRNA splicing. A StuI restriction fragment length polymorphism detected three alleles among various rat strains using this PCR S(A) gene probe. Alleles at the S(A) locus strongly cosegregated with blood pressure (P = .0012) in a salt-fed F2 population derived from crossing Dah1 S and Lewis rats. In contrast, a cosegregation analysis of blood pressure and S(A) alleles in salt-fed F2 rats derived from a Dah1 S X Wistar-Kyoto cross gave a negative cosegregation result. It is concluded that in certain genetic backgrounds the S(A) gene (or a closely linked gene) can contribute a significant component of genetic hypertension.

引用

页码：330 / 334

页数：5

共 15 条

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