PCR DETECTION OF TI AND RI PLASMIDS FROM PHYTOPATHOGENIC AGROBACTERIUM STRAINS

被引：97

作者：

SAWADA, H ^{[1
]}

IEKI, H ^{[1
]}

MATSUDA, I ^{[1
]}

机构：

[1] FRUIT TREE RES STN,OKITSU BRANCH,SHIMIZU,SHIZUOKA 42402,JAPAN

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1995年 / 61卷 / 02期

关键词：

D O I：

10.1128/AEM.61.2.828-831.1995

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A universal primer set (VCF/VCR) for PCR analysis based on the sequences of the virC operon located on Ti and Ri plasmids was designed to detect these plasmids from phytopathogenic Agrobacterium strains. With the VCF (sequence, 5'-ATCATTTGTAGCGACT-3') and VCR (sequence, 5'-AGCTCAAACCTGCTTC-3') primer set, DNA fragments of 730 bp in length were amplified from cell lysates of 10 rhizogenic and 65 tumorigenic agrobacteria. DNA sequencing and Southern hybridization analysis confirmed that the amplified fragments corresponded to the target region. The PCR method is considered convenient for routine determination of the potential pathogenicity of Agrobacterium strains.

引用

页码：828 / 831

页数：4

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