Identification of the Chromatin Regions Coated by Non-coding Xist RNA

被引：19

作者：

Murakami, K. ^{[1
,2
]}

Ohhira, T. ^{[1
]}

Oshiro, E. ^{[1
]}

Qi, D. ^{[1
]}

Oshimura, M. ^{[1
]}

Kugoh, H. ^{[1
]}

机构：

[1] Tottori Univ, Grad Sch Med Sci, Dept Biomed Sci Regenerat Med & Biofunct, Yonago, Tottori 6838503, Japan

[2] CDB, RIKEN, Lab Pluripotent Cell Studies, Kobe, Hyogo, Japan

来源：

CYTOGENETIC AND GENOME RESEARCH | 2009年 / 125卷 / 01期

基金：

日本学术振兴会;

关键词：

ckIP; FISH; LIT1/KCNQ1OT1; ncRNA; RNA; X chromosome; XCI; BECKWITH-WIEDEMANN-SYNDROME; X-CHROMOSOME INACTIVATION; GENE-EXPRESSION; DOSAGE COMPENSATION; METHYLATION; TRANSCRIPT; MAMMALS; PROFILE; CANCER; LOCUS;

D O I：

10.1159/000207514

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Xist non-coding RNA (ncRNA) is essential for X chromosome inactivation (XCI). Some genes can escape from XCI, but how this occurs is unknown. We developed a modified RNA tagging and recovery of associated proteins ( TRAP) method to study the association between Xist RNA and its target genes. In mouse cells, Xist RNA was detected on the Uba1 gene, but not on Jarid1c and Utx genes, which escape from XCI. Using this technique we were able to show that the Xist RNA molecule is not present on active genes that escape from XCI, but is present on genes inactivated by XCI, suggesting that this method is a powerful tool for functional analysis of ncRNA. Copyright (C) 2009 S. Karger AG, Basel

引用

页码：19 / 25

页数：7

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