TISSUE-SPECIFIC EXPRESSION OF THE TMV COAT PROTEIN IN TRANSGENIC TOBACCO PLANTS AFFECTS THE LEVEL OF COAT PROTEIN-MEDIATED VIRUS PROTECTION

被引:13
作者
CLARK, WG
REGISTER, JC
NEJIDAT, A
EICHHOLTZ, DA
SANDERS, PR
FRALEY, RT
BEACHY, RN
机构
[1] WASHINGTON UNIV,DEPT BIOL,ST LOUIS,MO 63130
[2] MONSANTO CO,ST LOUIS,MO 63198
[3] GARST RES,SLATER,IA 50244
关键词
D O I
10.1016/0042-6822(90)90131-A
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Transgenic tobacco plants were produced that express a chimeric gene encoding the coat protein (CP) of tobacco mosaic virus (TMV) under the control of the promoter from a ribulose bisphosphate carboxylase small subunit (rbcS) gene. Plant lines expressing comparable levels of CP from the rbcS and cauliflower mosaic virus 35S promoters were compared for resistance to TMV. In whole plant assays the 35S:CP constructs gave higher resistance than the rbcS:CP constructs. On the other hand, leaf mesophyll protoplasts isolated from both plant lines were equally resistant to infection by TMV. This indicated that the difference in resistance between the lines in the whole plant assay reflects differences at the level of short- and/or long-distance spread of TMV. Therefore, we propose that the difference in tissue-specific expression between the 35S and rbcS promoters accounts for greater resistance in the plant lines that express the 35S:CP chimeric genes. © 1990.
引用
收藏
页码:640 / 647
页数:8
相关论文
共 28 条
[1]   DELAY OF DISEASE DEVELOPMENT IN TRANSGENIC PLANTS THAT EXPRESS THE TOBACCO MOSAIC-VIRUS COAT PROTEIN GENE [J].
ABEL, PP ;
NELSON, RS ;
DE, B ;
HOFFMANN, N ;
ROGERS, SG ;
FRALEY, RT ;
BEACHY, RN .
SCIENCE, 1986, 232 (4751) :738-743
[2]   CHARACTERIZATION OF A 2ND PROTEIN ASSOCIATED WITH VIRIONS OF TOBACCO MOSAIC-VIRUS [J].
ASSELIN, A ;
ZAITLIN, M .
VIROLOGY, 1978, 91 (01) :173-181
[3]   COAT PROTEIN-MEDIATED RESISTANCE AGAINST VIRUS-INFECTION [J].
BEACHY, RN ;
LOESCHFRIES, S ;
TUMER, NE .
ANNUAL REVIEW OF PHYTOPATHOLOGY, 1990, 28 :451-474
[4]   REGULATED GENES IN TRANSGENIC PLANTS [J].
BENFEY, PN ;
CHUA, NH .
SCIENCE, 1989, 244 (4901) :174-181
[5]  
Clark M.F., 1984, METHODS VIROLOGY, P51, DOI DOI 10.1016/B978-0-12-470207-3.50009-7
[6]   VIRAL PROTECTION IN TRANSGENIC TOBACCO PLANTS EXPRESSING THE CUCUMBER MOSAIC-VIRUS COAT PROTEIN OR ITS ANTISENSE RNA [J].
CUOZZO, M ;
OCONNELL, KM ;
KANIEWSKI, W ;
FANG, RX ;
CHUA, NH ;
TUMER, NE .
BIO-TECHNOLOGY, 1988, 6 (05) :549-&
[7]  
GOWTOW K, 1984, PLANT CELL PHYSL, V25, P671
[8]   ANALYSIS OF THE MECHANISM OF PROTECTION IN TRANSGENIC PLANTS EXPRESSING THE POTATO VIRUS-X COAT PROTEIN OR ITS ANTISENSE RNA [J].
HEMENWAY, C ;
FANG, RX ;
KANIEWSKI, WK ;
CHUA, NH ;
TUMER, NE .
EMBO JOURNAL, 1988, 7 (05) :1273-1280
[9]   THE GENETIC-ENGINEERING OF 2 COMMERCIAL POTATO CULTIVARS FOR RESISTANCE TO POTATO VIRUS-X [J].
HOEKEMA, A ;
HUISMAN, MJ ;
MOLENDIJK, L ;
VANDENELZEN, PJM ;
CORNELISSEN, BJC .
BIO-TECHNOLOGY, 1989, 7 (03) :273-278
[10]   A SIMPLE AND GENERAL-METHOD FOR TRANSFERRING GENES INTO PLANTS [J].
HORSCH, RB ;
FRY, JE ;
HOFFMANN, NL ;
EICHHOLTZ, D ;
ROGERS, SG ;
FRALEY, RT .
SCIENCE, 1985, 227 (4691) :1229-1231