DEVELOPMENT OF A MONOCLONAL-BASED ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR THE COCCIDIOSTAT SALINOMYCIN

The polyether ionophoric coccidiostats are an important class of antimicrobials because of their extensive global use in modern agriculture. Salinomycin, the most widely used ionophoric anticoccidial in the U.S. broiler industry, has been marketed in all of the poultry-producing countries of the world. Established methods of analysis of salinomycin have disadvantages limiting their utility. We report here the development of 16 monoclonal anti-salinomycin antibodies. These monoclonal antibodies were used in competition enzyme-linked immunosorbent assays to differentially recognize salinomycin and the structurally similar narasin in the 10 ppb range. A preliminary immunoassay method has been developed, using one anti-salinomycin monoclonal antibody (SAL05), for quantization of salinomycin in poultry liver. The immunoassay gives a linear response with a good correlation between the spiked and observed values.