OPERATING CHARACTERISTICS OF A PREPARATIVE-SCALE RADIAL COMPRESSION CHROMATOGRAPH IN ISOCRATIC AND GRADIENT ELUTION

被引：10

作者：

CARTA, G

STRINGFIELD, WB

机构：

[1] Center for Bioprocess Development, Department of Chemical Engineering, University of Virginia, Charlottesville

来源：

JOURNAL OF CHROMATOGRAPHY A | 1994年 / 658卷 / 02期

关键词：

D O I：

10.1016/0021-9673(94)80031-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The performance of a preparative-scale radial compression chromatographic unit was studied for a reversed-phase model separation with commercial packings. The effects of radial compression on column back-pressure, peak shape, efficiency and column stability were studied for isocratic and gradient elution. The application of radial compression was found to be beneficial, allowing the column to be operated with efficiencies comparable to those obtained with analytical-scale equipment and stably over long periods of time.

引用

页码：407 / 417

页数：11

共 15 条

[1] ANALYTIC SOLUTION FOR VOLUME-OVERLOADED GRADIENT ELUTION CHROMATOGRAPHY [J].

CARTA, G ;

STRINGFIELD, WB .

JOURNAL OF CHROMATOGRAPHY, 1992, 605 (02) :151-159

[2]

COLIN H, 1990, LC GC-MAG SEP SCI, V8, P302

[3]

COLIN H, 1993, PREPARATIVE PRODUCTI, P11

[4]

COX GB, 1990, LC GC-MAG SEP SCI, V8, P690

[5]

Giddings J. C., 1991, UNIFIED SEPARATION S

[6] USE OF AN 18-MM ID COLUMN FOR ANALYTICAL-SCALE AND SEMI-PREPARATIVE-SCALE HIGH-PRESSURE LIQUID-CHROMATOGRAPHY [J].

GODBILLE, E ;

DEVAUX, P .

JOURNAL OF CHROMATOGRAPHY, 1976, 122 (JUL7) :317-329

[7] PRACTICAL ASPECTS OF LC THEORY [J].

KNOX, JH .

JOURNAL OF CHROMATOGRAPHIC SCIENCE, 1977, 15 (09) :352-364

[8] PREPARATIVE LIQUID-CHROMATOGRAPHY USING RADIALLY COMPRESSED COLUMNS [J].

LITTLE, JN ;

COTTER, RL ;

PRENDERGAST, JA ;

MCDONALD, PD .

JOURNAL OF CHROMATOGRAPHY, 1976, 126 (NOV3) :439-445

[9]

NICOUD RM, 1993, PREPARATIVE PRODUCTI, P47

[10]

Snyder L. R., 1980, HIGH PERFORMANCE LIQ, V1, P208

← 1 2 →