MECHANISM OF CDK ACTIVATION REVEALED BY THE STRUCTURE OF A CYCLINA-CDK2 COMPLEX

被引：1210

作者：

JEFFREY, PD

RUSO, AA

POLYAK, K

GIBBS, E

HURWITZ, J

MASSAGUE, J

PAVLETICH, NP

机构：

[1] MEM SLOAN KETTERING CANC CTR,CELL BIOL & GENET PROGRAM,NEW YORK,NY 10021

[2] MEM SLOAN KETTERING CANC CTR,HOWARD HUGHES MED INST,NEW YORK,NY 10021

[3] MEM SLOAN KETTERING CANC CTR,PROGRAM MOLEC BIOL,NEW YORK,NY 10021

来源：

NATURE | 1995年 / 376卷 / 6538期

关键词：

D O I：

10.1038/376313a0

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The crystal structure of the human cyclinA-cyclin-dependent kinase2 (CDK2)-ATP complex has been determined at 2.3 Angstrom resolution. CyclinA binds to one side of CDK2's catalytic cleft, inducing large conformational changes in its PSTAIRE helix and T-loop. These changes activate the kinase by realigning active site residues and relieving the steric blockade at the entrance of the catalytic cleft.

引用

页码：313 / 320

页数：8

共 50 条

[1] BRUNGER AT, 1991, XPLOR SYSTEM CRYSTAL
[2] PHOSPHORYLATION INDEPENDENT ACTIVATION OF HUMAN CYCLIN-DEPENDENT KINASE-2 BY CYCLIN-A INVITRO
CONNELLCROWLEY, L
SOLOMON, MJ
WEI, N
HARPER, JW
[J]. MOLECULAR BIOLOGY OF THE CELL, 1993, 4 (01) : 79 - 92
[3] ANTIBODY-ANTIGEN COMPLEXES
DAVIES, DR
PADLAN, EA
SHERIFF, S
[J]. ANNUAL REVIEW OF BIOCHEMISTRY, 1990, 59 : 439 - 473
[4] CRYSTAL-STRUCTURE OF CYCLIN-DEPENDENT KINASE-2
DEBONDT, HL
ROSENBLATT, J
JANCARIK, J
JONES, HD
MORGAN, DO
KIM, SH
[J]. NATURE, 1993, 363 (6430) : 595 - 602
[5] ACTIVATION OF HUMAN CYCLIN-DEPENDENT KINASES INVITRO
DESAI, D
GU, Y
MORGAN, DO
[J]. MOLECULAR BIOLOGY OF THE CELL, 1992, 3 (05) : 571 - 582
[6] CDC2 PHOSPHORYLATION IS REQUIRED FOR ITS INTERACTION WITH CYCLIN
DUCOMMUN, B
BRAMBILLA, P
FELIX, MA
FRANZA, BR
KARSENTI, E
DRAETTA, G
[J]. EMBO JOURNAL, 1991, 10 (11) : 3311 - 3319
[7] DIFFERENTIAL REGULATION OF E2F TRANSACTIVATION BY CYCLIN CDK2 COMPLEXES
DYNLACHT, BD
FLORES, O
LEES, JA
HARLOW, E
[J]. GENES & DEVELOPMENT, 1994, 8 (15) : 1772 - 1786
[8] FUNCTIONAL INTERACTIONS OF THE RETINOBLASTOMA PROTEIN WITH MAMMALIAN D-TYPE CYCLINS
EWEN, ME
SLUSS, HK
SHERR, CJ
MATSUSHIME, H
KATO, JY
LIVINGSTON, DM
[J]. CELL, 1993, 73 (03) : 487 - 497
[9] A NOVEL CYCLIN ASSOCIATES WITH MO15/CDK7 TO FORM THE CDK-ACTIVATING KINASE
FISHER, RP
MORGAN, DO
[J]. CELL, 1994, 78 (04) : 713 - 724
[10] MERLOT, AN INTEGRATED PACKAGE OF COMPUTER-PROGRAMS FOR THE DETERMINATION OF CRYSTAL-STRUCTURES BY MOLECULAR REPLACEMENT
FITZGERALD, PMD
[J]. JOURNAL OF APPLIED CRYSTALLOGRAPHY, 1988, 21 (03) : 273 - 278

← 1 2 3 4 5 →