ADAPTATION OF SPODOPTERA-EXIGUA LARVAE TO PLANT PROTEINASE-INHIBITORS BY INDUCTION OF GUT PROTEINASE ACTIVITY INSENSITIVE TO INHIBITION

被引:358
作者
JONGSMA, MA
BAKKER, PL
PETERS, J
BOSCH, D
STIEKEMA, WJ
机构
[1] Department of Molecular Biology, DLO-Ctr. Plant Breed. Repro. Res., 6700 AA Wageningen
关键词
INSECT RESISTANCE; POTATO PROTEINASE INHIBITOR II; TRANSGENIC PLANT; WOUND INDUCTION;
D O I
10.1073/pnas.92.17.8041
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Tobacco plants were transformed with a cDNA clone of chymotrypsin/trypsin-specific potato proteinase inhibitor II (PI2) under the control of a constitutive promoter. Although considerable levels of transgene expression could be demonstrated, the growth of Spodoptera exigua larvae fed with detached leaves of PI2-expressing plants was not affected. Analysis of the composition of tryptic gut activity demonstrated that only 18% of the proteinase activity of insects reared on these transgenic plants was sensitive to inhibition by PI2, whereas 78% was sensitive in insects reared on control plants. Larvae had compensated for this loss of tryptic activity by a 2.5-fold induction of new activity that was insensitive to inhibition by PI2. PI2-insensitive proteolytic activity was also induced in response to endogenous proteinase inhibitors of tobacco; therefore, induction of such proteinase activity may represent the mechanism by which insects that feed on plants overcome plant proteinase inhibitor defense.
引用
收藏
页码:8041 / 8045
页数:5
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