GENETIC DIVERSITY AND HOST RANGE IN STRAINS OF ERWINIA-CHRYSANTHEMI

被引：38

作者：

BOCCARA, M

VEDEL, R

LALO, D

LEBRUN, MH

LAFAY, JF

机构：

[1] Laboratoire de Pathologie Vegetale, INRA, 75005 Paris

[2] IRAT-CIRAD Martinique

[3] Cryptogamie, Universite Paris 11, 91405 Orsay, Cedex

来源：

MOLECULAR PLANT-MICROBE INTERACTIONS | 1991年 / 4卷 / 03期

关键词：

PECTATE LYASE; PECTIN METHYL ESTERASE; SOFT ROT;

D O I：

10.1094/MPMI-4-293

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Restriction fragment length polymorphism (RFLP) analysis was used to study the relationships among 52 strains of Erwinia chrysanthemi differing in such factors as original host, geographical origin, and year of isolation. The strains were distributed in 10 RFLP groups. The similarity coefficients obtained with a probe encoding pathogenicity genes of E. chrysanthemi gave similar results compared to those obtained with randomly selected probes from a cosmid library of E. chrysanthemi 3937. Comparison with other classification systems, such as biovars and pectinolytic enzyme profiles, showed that RFLP analysis was the most discriminative. However, good correlation between these methods was observed when very homogenous groups of strains were analyzed. A sample of strains from different RFLP groups was assayed for pathogenicity on different plant species. Some host plants, such as potato, tomato, lettuce, and saintpaulia, were susceptible to all the tested strains of E. chrysanthemi. Others, such as dieffenbachia and philodendron, were differential hosts because they were only attacked by members of particular RFLP groups.

引用

页码：293 / 299

页数：7

共 29 条

[1]

Aymeric J.L., Enard C., Renou F., Neema C., Boccara M., Expert D., The requirement of depolymerizing enzymes and a high affinity iron transport system for pathogenicity of Erwinia chrysanthemi, Proc. Int. Conf. Plant Pathog. Bact., 7th. Budapest, pp. 679-683, (1990)

[2]

Bertheau Y., Madgidi-Hervan E., Kotoujansky A., Detection of depolymerase isoenzymes after electrophoresis or electrofocusing, or in titration curves, Analytical Biochemistry, 139, 2, pp. 383-389, (1984)

[3]

Boccara M., Diolez A., Rouve M., Kotoujansky A., The role of individual pectate lyases of Erwinia chrysanthemi 3937 in pathogenicity on saintpaulia plants, Physiol. Mol. Plant Pathol., 33, pp. 95-104, (1988)

[4]

Bradbury J.F., Guide to plant pathogenic bacteria, Commonw. Agric. Bur. Annot. Bibliogr. Int. Mycol. Inst./Assoc. Appl. Biol., pp. 72-76, (1984)

[5]

Cook D., Barlow E., Sequeira L., Genetic diversity of Pseudo-monas solanacearum: Detection of restriction fragment length polymorphisms with DNA probes that specify virulence and the hypersensitive response, Mol. Plant-microbe Interact, 2, pp. 113-121, (1989)

[6]

De Parasis J., Roth D.A., Nucleic acid probes for identification of phytobacteria: Identification of genus-specific 16S rRNA sequences, Phytopathology, 80, pp. 618-621, (1990)

[7]

Dickey R.S., Erwinia chrysanthemi'. Reaction of eight plant species to strains from several hosts and to strains of other Erwinia species, Phytopathology, 71, pp. 23-29, (1981)

[8]

Dickey R.S., Victoria J.I., Taxonomy and emended description of strains of Erwinia isolated from Musa paradisiaca linnaeus, International Journal of Systematic Bacteriology, 30, 1, pp. 129-134, (1980)

[9]

Enard C., Diolez A., Expert D., Systemic virulence in Erwinia chrysanthemi requires a functional high affinity iron assimilation system, J. Bacteriol, 170, pp. 2419-2426, (1988)

[10]

Fitch W.M., Margoliash E., Construction of phylogenetic trees, Science, 155, pp. 279-284, (1967)

← 1 2 3 →