MODIFIED METHODS FOR SIMPLE AND RAPID DETECTION OF BACTERIAL DEOXYRIBONUCLEASE PRODUCTION

被引：6

作者：

CHAUDHURI, P ^{[1
]}

SINGH, SD ^{[1
]}

机构：

[1] INDIAN VET RES INST,NATL BIOTECHNOL CTR,GENET ENGN BACTERIA LAB,IZATNAGAR 243122,UTTAR PRADESH,INDIA

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 1992年 / 16卷 / 04期

关键词：

DEOXYRIBONUCLEIC ACID; DEOXYRIBONUCLEASE; DEPOLYMERIZATION; METHYL GREEN; TOLUIDINE BLUE-O;

D O I：

10.1016/0167-7012(92)90083-G

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Simple, rapid and sensitive tests for the detection of deoxyribonuclease activity are described. The modified methyl green-DNA agar and toluidine blue O-DNA agar tests were performed in simple glass slides using the respective dye (methyl green and toluidine blue O) as test reagent. Positive tests of methyl green-DNA agar produced a clear zone against green background and toluidine blue O-DNA agar produced a pink zone in a royal blue background in 2-6 h. Modified methods are sensitive to detect as little as 10-20 ng of DNase.

引用

页码：307 / 311

页数：5

共 14 条

[1] TURBIDIMETRIC ASSAY OF STAPHYLOCOCCAL NUCLEASE [J].