TRANSCRIPTION FACTOR NUCLEAR FACTOR-I PROTEINS FORM STABLE HOMODIMERS AND HETERODIMERS

被引：117

作者：

KRUSE, U ^{[1
]}

SIPPEL, AE ^{[1
]}

机构：

[1] UNIV FREIBURG,INST BIOL GENET 3,D-79104 FREIBURG,GERMANY

来源：

FEBS LETTERS | 1994年 / 348卷 / 01期

关键词：

TRANSCRIPTION FACTOR; DNA BINDING; DIMERIZATION; NUCLEAR FACTOR I; TGGCA PROTEIN; CCAAT BINDING TRANSCRIPTION FACTOR;

D O I：

10.1016/0014-5793(94)00585-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nuclear factor I (NFI) proteins constitute a large family of eukaryotic DNA binding proteins. They are involved in viral and cellular aspects of transcriptional regulation and they are capable of stimulating adenovirus initiation of replication. Using in vitro translated NFI proteins encoded by four different chicken NFI genes, we have detected homodimers as well as heterodimers for all combinations tested. The formation of heterodimers was critically dependent on cotranslation, indicating stable dimer formation in the absence of DNA. The unrestricted heterodimerization of NFI proteins adds, beside gene diversity and alternative splicing, another level of diversity to this protein family.

引用

页码：46 / 50

页数：5

共 24 条

[1] A STRATEGY FOR EFFICIENT INVITRO TRANSLATION OF CDNAS USING THE RABBIT BETA-GLOBIN LEADER SEQUENCE [J].

ANNWEILER, A ;

HIPSKIND, RA ;

WIRTH, T .

NUCLEIC ACIDS RESEARCH, 1991, 19 (13) :3750-3750

[2] THE TGGCA-BINDING PROTEIN - A EUKARYOTIC NUCLEAR-PROTEIN RECOGNIZING A SYMMETRICAL SEQUENCE ON DOUBLE-STRANDED LINEAR DNA [J].

BORGMEYER, U ;

NOWOCK, J ;

SIPPEL, AE .

NUCLEIC ACIDS RESEARCH, 1984, 12 (10) :4295-4311

[3] MULTIPLE GENES ENCODE NUCLEAR FACTOR-1-LIKE PROTEINS THAT BIND TO THE PROMOTER FOR 3-HYDROXY-3-METHYLGLUTARYL-COENZYME-A REDUCTASE [J].

GIL, G ;

SMITH, JR ;

GOLDSTEIN, JL ;

SLAUGHTER, CA ;

ORTH, K ;

BROWN, MS ;

OSBORNE, TF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (23) :8963-8967

[4] AMINO-TERMINAL DOMAIN OF NF1 BINDS TO DNA AS A DIMER AND ACTIVATES ADENOVIRUS DNA-REPLICATION [J].

GOUNARI, F ;

DEFRANCESCO, R ;

SCHMITT, J ;

VANDERVLIET, PC ;

CORTESE, R ;

STUNNENBERG, H .

EMBO JOURNAL, 1990, 9 (02) :559-566

[5] IDENTIFICATION OF A POTENT ADIPOCYTE-SPECIFIC ENHANCER - INVOLVEMENT OF AN NF-1-LIKE FACTOR [J].

GRAVES, RA ;

TONTONOZ, P ;

ROSS, SR ;

SPIEGELMAN, BM .

GENES & DEVELOPMENT, 1991, 5 (03) :428-437

[6] THE -6.1-KILOBASE CHICKEN LYSOZYME ENHANCER IS A MULTIFACTORIAL COMPLEX CONTAINING SEVERAL CELL-TYPE-SPECIFIC ELEMENTS [J].

GREWAL, T ;

THEISEN, M ;

BORGMEYER, U ;

GRUSSENMEYER, T ;

RUPP, RAW ;

STIEF, A ;

QIAN, F ;

HECHT, A ;

SIPPEL, AE .

MOLECULAR AND CELLULAR BIOLOGY, 1992, 12 (05) :2339-2350

[7] CHARACTERIZATION OF JUND - A NEW MEMBER OF THE JUN PROTO-ONCOGENE FAMILY [J].

HIRAI, SI ;

RYSECK, RP ;

MECHTA, F ;

BRAVO, R ;

YANIV, M .

EMBO JOURNAL, 1989, 8 (05) :1433-1439

[8] GCN4, A EUKARYOTIC TRANSCRIPTIONAL ACTIVATOR PROTEIN, BINDS AS A DIMER TO TARGET DNA [J].

HOPE, IA ;

STRUHL, K .

EMBO JOURNAL, 1987, 6 (09) :2781-2784

[9] O-GLYCOSYLATION OF EUKARYOTIC TRANSCRIPTION FACTORS - IMPLICATIONS FOR MECHANISMS OF TRANSCRIPTIONAL REGULATION [J].

JACKSON, SP ;

TJIAN, R .

CELL, 1988, 55 (01) :125-133

[10] THE GENES FOR TRANSCRIPTION FACTOR NUCLEAR FACTOR-I GIVE RISE TO CORRESPONDING SPLICE VARIANTS BETWEEN VERTEBRATE SPECIES [J].

KRUSE, U ;

SIPPEL, AE .

JOURNAL OF MOLECULAR BIOLOGY, 1994, 238 (05) :860-865

← 1 2 3 →