FLAVIVIRUS TYPE-SPECIFIC ANTIGENS PRODUCED FROM FUSIONS OF A PORTION OF THE E-PROTEIN GENE WITH THE ESCHERICHIA-COLI TRPE GENE

被引：28

作者：

FONSECA, BAL

KHOSHNOOD, K

SHOPE, RE

MASON, PW

机构：

[1] YALE UNIV, SCH MED, DEPT EPIDEMIOL & PUBL HLTH, YALE ARBOVIRUS RES UNIT, NEW HAVEN, CT 06510 USA

[2] INST ADOLFO LUTZ REGISTRO, DEPT VIROL, SECAO ARBOVIRUS, SAO PAULO, BRAZIL

[3] USDA ARS, PLUM ISL ANIM DIS CTR, GREENPORT, NY 11944 USA

来源：

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE | 1991年 / 44卷 / 05期

关键词：

D O I：

10.4269/ajtmh.1991.44.500

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

Based on previous data showing that JEV and DEN-1 fusion proteins can be used as antigens, we engineered analogous fusion proteins for the same regions of the E proteins of DEN-2, DEN-3, and DEN-4, using the polymerase chain reaction. The resulting fusion proteins were tested in a modified ELISA for their reactivity with mouse immune ascitic fluids (MIAF) generated against 10 different flaviviruses. The results showed that these recombinant antigens could be used as type-specific immunological reagents, and suggest that these antigens could ser-ve as the basis for rapid, safe, and inexpensive flavivirus serosurveys.

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页码：500 / 508

页数：9

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