MOLECULAR-CLONING AND EXPRESSION OF CHICK GAL-BETA-1,3GALNAC ALPHA-2,3-SIALYLTRANSFERASE

被引：22

作者：

KUROSAWA, N ^{[1
]}

HAMAMOTO, T ^{[1
]}

INOUE, M ^{[1
]}

TSUJI, S ^{[1
]}

机构：

[1] INST PHYS & CHEM RES,FRONTIER RES PROGRAM,WAKO,SAITAMA 35101,JAPAN

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS | 1995年 / 1244卷 / 01期

关键词：

SIALYLTRANSFERASE; CHICK EMBRYO; CDNA CLONING;

D O I：

10.1016/0304-4165(95)00012-Z

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cDNA clone encoding chick Gal beta 1,3GalNAc alpha 2,3-sialyltransferase (ST3Gal I) was isolated from a chick embryo brain cDNA library. The cDNA sequence included an open reading frame coding for 342 amino acids, and the deduced amino acid sequence showed 64% identity with that of the mouse enzyme. Northern blot analysis of chick embryos revealed that the ST3Gal I gene was expressed in early embryonic stages. The identity of the enzyme was confirmed by construction of a recombinant sialyltransferase in which the N-terminal part including the cytoplasmic tail and signal anchor domain was replaced with an immunoglobulin signal peptide sequence. This enzyme expressed in COS-7 cells exhibited transferase activity similar to that of mouse ST3Gal I.

引用

页码：216 / 222

页数：7

共 36 条

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