THE MOLECULAR-BIOLOGY OF THE FLAVIN-CONTAINING MONOOXYGENASES OF MAN

被引：125

作者：

PHILLIPS, IR

DOLPHIN, CT

CLAIR, P

HADLEY, MR

HUTT, AJ

MCCOMBIE, RR

SMITH, RL

SHEPHARD, EA

机构：

[1] UNIV LONDON KINGS COLL,DEPT PHARM,LONDON SW3 6LX,ENGLAND

[2] UNIV LONDON UNIV COLL,DEPT BIOCHEM & MOLEC BIOL,LONDON WC1E 6BT,ENGLAND

[3] UNIV LONDON IMPERIAL COLL SCI TECHNOL & MED,ST MARYS HOSP,SCH MED,DEPT PHARMACOL & TOXICOL,LONDON W2 1PG,ENGLAND

来源：

CHEMICO-BIOLOGICAL INTERACTIONS | 1995年 / 96卷 / 01期

基金：

英国惠康基金;

关键词：

FLAVIN-CONTAINING MONOOXYGENASES; CDNA CLONING; CHROMOSOMAL LOCALIZATION; GENE REGULATION; EXPRESSION; STEREOSELECTIVITY;

D O I：

10.1016/0009-2797(94)03580-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

cDNA clones encoding five distinct members of the FMO family of man (FMOs 1, 2, 3, 4 and 5) were isolated by a combination of library screening and reverse transcription-polymerase chain reaction techniques. The deduced amino acid sequences of the human FMOs have 82-87% identity with their known orthologues in other mammals but only 51-57% similarity to each other. The hydropathy profiles of the proteins are very similar. From the calculated rate of evolution of FMOs (a 1% change in sequence per 6 million years) it would appear that individual members of the FMO gene family arose by duplication of a common ancestral gene some 250-300 million years ago. Each of the FMO genes was mapped by the polymerase chain reaction to the long arm of human chromosome 1. The localization of the FMO1 gene was further refined to lq23-q25 by in situ hybridization of human

引用

页码：17 / 32

页数：16

共 36 条

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