ASSAY OF PENICILLIN ACYLASE IN ORGANIC MEDIA

被引：4

作者：

ALVES, JRS

FONSECA, LP

RAMALHO, MT

CABRAL, JMS

机构：

[1] UNIV MINHO, DEPT QUIM, P-4700 BRAGA, PORTUGAL

[2] INST SUPER TECN, ENGN BIOQUIM LAB, P-1000 LISBON, PORTUGAL

来源：

BIOTECHNOLOGY TECHNIQUES | 1995年 / 9卷 / 10期

关键词：

D O I：

10.1007/BF00159238

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The synthetic substrate 6-nitro-3-(phenylacetamido) benzoic acid (NIPAB) is an appropriate substrate for assaying penicillin acylase activity in reversed micellar systems of Aerosol - OT in isooctane. Accumulation of 6-nitro-3-aminobenzoic acid (NABA) produced by the enzymatic hydrolysis of NIPAB, followed by the increase in absorbance at 405 nm, was linear at 4 to 20 mM for up to 30 minutes and 15 degrees C to 40 degrees C.

引用

页码：729 / 730

页数：2

共 5 条

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[2]

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[4]

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[5]

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