DEVELOPMENT AND EVALUATION OF A RAPID ABSORBED ENZYME-IMMUNOASSAY TEST FOR THE DIAGNOSIS OF JOHNES DISEASE IN CATTLE

被引:78
作者
COX, JC [1 ]
DRANE, DP [1 ]
JONES, SL [1 ]
RIDGE, S [1 ]
MILNER, AR [1 ]
机构
[1] VICTORIAN INST ANIM SCI,DEPT AGR & RURAL AFFAIRS,WESTMEADOWS,VIC 3049,AUSTRALIA
关键词
D O I
10.1111/j.1751-0813.1991.tb03167.x
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
An absorbed enzyme immunoassay (EIA) test for Johne's disease in cattle was developed in which absorption of cross-reacting antibodies occurred as a rapid reaction in solution rather than overnight with whole organisms and a subsequent centrifugation step. Total test time was reduced to less than 2 h with a minimum of manipulations. The test was evaluated in cattle herds from Johne's disease-endemic and Johne's disease-free regions of Australia. Specificity was 99.8%. Calculations of sensitivity were affected by the history of the herd under test. However, the EIA detected in excess of 80% of animals before onset of clinical disease and 65% of faecal shedders were EIA positive on, or before, first detection of Mycobacterium paratuberculosis in their faeces. The test should aid epidemiological studies and be a useful tool in the management and control of Johne's disease.
引用
收藏
页码:157 / 160
页数:4
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