RAPID-DETERMINATION OF NUCLEOTIDES THAT DEFINE TRANSFER-RNA GLY ACCEPTOR IDENTITY

被引：68

作者：

MCCLAIN, WH

FOSS, K

JENKINS, RA

SCHNEIDER, J

机构：

[1] Department of Bacteriology, University of Wisconsin, Madison

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1991年 / 88卷 / 14期

关键词：

D O I：

10.1073/pnas.88.14.6147

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Expression of the genetic code depends on the recognition of specific tRNAs by the enzymes that aminoacylate them. A computer comparison of tRNA sequences coupled with analysis of mutant nonsense-suppressor tRNAs has revealed the structural features that distinguish the acceptor identity of Escherichia coli tRNA(Gly) from tRNAs that accept phenylalanine, arginine, lysine, and glutamine. On replacement of several nucleotides in the acceptor stem and anticodon of the latter tRNAs with tRNA(Gly)-derived residues, the resulting molecules acquired a tRNA(Gly) identity.

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页码：6147 / 6151

页数：5

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