ENZYME-CATALYZED CARBON CARBON BOND FORMATION - USE OF TRANSKETOLASE FROM ESCHERICHIA-COLI

被引：47

作者：

HOBBS, GR

LILLY, MD

TURNER, NJ

WARD, JM

WILLETS, AJ

WOODLEY, JM

机构：

[1] UNIV EXETER,DEPT CHEM,STOCKER RD,EXETER EX4 4RJ,DEVON,ENGLAND

[2] UNIV LONDON UNIV COLL,DEPT CHEM & BIOCHEM ENGN,ADV CTR BIOCHEM ENGN,LONDON WC1E 7JE,ENGLAND

[3] UNIV LONDON UNIV COLL,DEPT BIOCHEM & MOLEC BIOL,LONDON WC1E 6BT,ENGLAND

[4] UNIV EXETER,DEPT BIOL SCI,EXETER EX4 4RJ,DEVON,ENGLAND

来源：

JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 1 | 1993年 / 02期

关键词：

D O I：

10.1039/p19930000165

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Transketolase has been obtained in greater quantities from an over-expressed E. coli transformant carrying the transketolase gene. Crude extracts of this organism are suitable for use in small scale biotransformations to provide mmol quantities of product. Initial results indicate that the transketolase from E. coli is relatively non-specific for the aldehyde component of the reaction.

引用

页码：165 / 166

页数：2

共 9 条

[1]

DATTA AG, 1961, J BIOL CHEM, V236, P617

[2] ENZYME-CATALYZED SYNTHESIS OF CARBOHYDRATES - SYNTHETIC POTENTIAL OF TRANSKETOLASE [J].