A HEAT-STABLE SERUM FACTOR UP-REGULATES 5-LIPOXYGENASE ACTIVITY IN HL-60 CELLS, MODULATION BY TNF-ALPHA OR GM-CSF

被引：8

作者：

STEINHILBER, D ^{[1
]}

RADMARK, O ^{[1
]}

SAMUELSSON, B ^{[1
]}

机构：

[1] UNIV TUBINGEN,DEPT PHARMACEUT CHEM,W-7400 TUBINGEN 1,GERMANY

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 193卷 / 03期

关键词：

D O I：

10.1006/bbrc.1993.1736

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

5-Lipoxygenase (5-LO) activity in differentiating HL-60 cells was upregulated by a heat stable protein in human serum. Hematopoietic cytokines were evaluated for this effect on 5-lipoxygenase activity, none of the compounds tested could replace serum. However, TNFα or GM-CSF were able to augment the effect of heat treated serum, giving 5-LO activities that (per cell) were higher than for granulocytes isolated from peripheral blood. The serum factor primarily upregulated 5-LO activity. The amounts of 5-LO protein, or mRNA for 5-LO or 5-lipoxygenase activating protein (FLAP) were less affected. © 1993 Academic Press, Inc.

引用

页码：1083 / 1090

页数：8

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