CLONING AND EXPRESSION OF CDNA-ENCODING A NEW-TYPE OF ASCORBATE PEROXIDASE FROM SPINACH

被引：55

作者：

ISHIKAWA, T ^{[1
]}

SAKAI, K ^{[1
]}

TAKEDA, T ^{[1
]}

SHIGEOKA, S ^{[1
]}

机构：

[1] KINKI UNIV,FAC AGR,DEPT FOOD & NUTR,NARA 631,JAPAN

来源：

FEBS LETTERS | 1995年 / 367卷 / 01期

关键词：

ASCORBATE PEROXIDASE; PEROXIDASE; CDNA CLONING; EXPRESSION; SPINACH;

D O I：

10.1016/0014-5793(95)00539-L

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cDNA clone (SAP1) encoding a peroxidase was isolated from a spinach cDNA library using monoclonal antibodies raised against Euglena ascorbate peroxidase. The deduced amino acid sequence of SAP1 had higher homology with the cytosolic ascorbate peroxidases from plant sources than with bacterial peroxidases and classical plant peroxidases. The peroxidase activity of recombinant SAP1 protein expressed in E. coli was 1.6-fold higher with ascorbate than with guaiacol, which was similar to those of endogenous cytosolic ascorbate peroxidases. Here me conclude that SAP1 belongs to a new type of ascorbate peroxidase from spinach.

引用

页码：28 / 32

页数：5

共 29 条

[1] ASCORBATE PEROXIDASE - A HYDROGEN PEROXIDE-SCAVENGING ENZYME IN PLANTS [J].