NEW STRATEGIES FOR THE DESIGN OF CATALYTIC ANTIBODIES

被引：25

作者：

JANDA, KD

机构：

[1] Department of Molecular Biology and Chemistry, Research Institute of Scripps Clinic, La Jolla, California, 92037

来源：

BIOTECHNOLOGY PROGRESS | 1990年 / 6卷 / 03期

关键词：

D O I：

10.1021/bp00003a002

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A new approach is advanced which is aimed at expanding the scope of antibody catalysis. It entails the design and synthesis of haptens that will elicit antibodies to catalyze acyl transfer reactions by a methodology which we term “bait and switch” catalysis. What we have done involves the placement of a point charge on the hapten in close proximity to, or in direct substitution for, a chemical functional group we wish to transform in the respective substrate. The haptenic charge should induce a complementary charge (on an amino acid residue) at the binding site. The substrate will lack this charge but will retain a similar overall structure. The monoclonal antibodies that bind these substrates now have the potential to act as general acids/bases for substrates having hydrolyzable functional groups. Copyright © 1990 American Institute of Chemical Engineers (AIChE)

引用

页码：178 / 181

页数：4

共 17 条

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