PURIFICATION OF MONOCLONAL-ANTIBODIES BY COMPLEX-DISPLACEMENT CHROMATOGRAPHY ON CM-CELLULOSE

被引：16

作者：

TORRES, AR

PETERSON, EA

机构：

[1] Bio-Fractionations, Logan, UT 84321

来源：

JOURNAL OF CHROMATOGRAPHY | 1990年 / 499卷

关键词：

D O I：

10.1016/S0021-9673(00)96962-0

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A single high-capacity chromatographic procedure was used to purify a monoclonal antibody from ascites fluid. A new displacement procedure involving a displacer protein complex is described. The scale-up from 1 ml to 450 ml of ascites sample was easily accomplished with this complex-displacement system. A monoclonal antibody recovery of 79% was measured for the largest-scale purification. © 1990.

引用

页码：47 / 54

页数：8

共 28 条

[1]

AKERSTROM B, 1986, J BIOL CHEM, V261, P240

[2]

BARNETT B, 1989, 6 INT C COMP APPL VI

[3] A ONE-STEP PURIFICATION METHOD FOR MONOCLONAL-ANTIBODIES BASED ON SALT-PROMOTED ADSORPTION CHROMATOGRAPHY ON A THIOPHILIC ADSORBENT [J].