INDOLE-3-ACETIC-ACID AND INDOLE-3-BUTYRIC ACID IN TISSUES OF CARROT INOCULATED WITH AGROBACTERIUM-RHIZOGENES

The role of auxins in induction of roots by Agrobacterium rhizogenes was studied in carrot root disks. Transformed roots were produced on root disks by inoculation with A. rhizogenes, A4. Measurement of indole-3-acetic acid (IAA) by gas chromatography-mass spectrometry (GC-MS) indicated that there was a significant increase in the concentration of IAA in transformed callus and induced roots compared with initial IAA concentrations in carrot disks. Indole-3-butyric acid (IBA) was found to occur naturally in carrot roots. The presence of IBA, a potent root inducer, must be taken into account when assessing the role of auxin during transformation and induction of roots by A. rhizogenes.