DERIVATIVES OF PUC18 THAT HAVE BGLII SITES FLANKING A MODIFIED MULTIPLE CLONING SITE AND THAT RETAIN THE ABILITY TO IDENTIFY RECOMBINANT CLONES BY VISUAL SCREENING OF ESCHERICHIA-COLI COLONIES

被引：203

作者：

JANSSEN, GR ^{[1
]}

BIBB, MJ ^{[1
]}

机构：

[1] JOHN INNES INST,JOHN INNES CTR,NORWICH NR4 7UH,NORFOLK,ENGLAND

来源：

GENE | 1993年 / 124卷 / 01期

关键词：

CLONING VECTORS; XGAL; ALPHA-COMPLEMENTATION; INSERTIONAL INACTIVATION;

D O I：

10.1016/0378-1119(93)90774-W

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We report the construction of a series of derivatives of pUC18 in which a modified multiple cloning site is flanked by one or two BglII sites. The reading frame of the lacZalpha fragment was retained in each construct, permitting the recognition of transformants containing plasmids with inserts by visual screening for loss of blue coloration.

引用

页码：133 / 134

页数：2

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