ISOLATION AND MAPPING OF A HUMAN GENE (MCM2) ENCODING A PRODUCT HOMOLOGOUS TO YEAST PROTEINS INVOLVED IN DNA-REPLICATION

被引：21

作者：

NAKATSURU, S ^{[1
]}

SUDO, K ^{[1
]}

NAKAMURA, Y ^{[1
]}

机构：

[1] SANKO JUNYAKU CO LTD,TOKYO,JAPAN

来源：

CYTOGENETICS AND CELL GENETICS | 1995年 / 68卷 / 3-4期

关键词：

D O I：

10.1159/000133918

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

From a human fetal-lung cDNA library, we isolated a gene, human MCM2, whose nucleotide sequence predicts a protein product homologous to the yeast nuclear proteins MCM2, MCM3, CDC21, and CDC46. Determination of cDNA sequences covering the entire coding region demonstrated an open reading frame of 1,629 nucleotides encoding 543 amino acids. Sequence comparison revealed 30-40% amino acid identity with these four yeast proteins, all of which are considered to play important roles in DNA replication. Analysis by Northern blotting revealed that the human MCM2 gene is expressed ubiquitously in normal tissues. Furthermore, we localized this gene to chromosomal bands 7q21.3-->q22.1 by fluorescence in situ hybridization.

引用

页码：226 / 230

页数：5

共 13 条

[1]

BREWER BJ, 1987, CELL, V51, P453

[2] CDC46/MCM5, A YEAST PROTEIN WHOSE SUBCELLULAR-LOCALIZATION IS CELL CYCLE-REGULATED, IS INVOLVED IN DNA-REPLICATION AT AUTONOMOUSLY REPLICATING SEQUENCES [J].