Analysis of culture fluorescence by a fiber-optic sensor in Nicotiana tabacum plant cell culture

被引：6

作者：

Choi, JW

Park, JH

Lee, SC

Kim, DI

Lee, WH

机构：

[1] Department of Chemical Engineering, Sogang University, Seoul

[2] Department of Biological Engineering, Inha University, Incheon

来源：

KOREAN JOURNAL OF CHEMICAL ENGINEERING | 1995年 / 12卷 / 05期

关键词：

Nicotiana tabacum; fiber-optic sensor; NADH fluorescence; dimensional analysis; structured kinetic model;

D O I：

10.1007/BF02705855

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The on-line sensing of viable cell weight in plant cell culture process is applied to analysis and control of process. The fiber-optic fluorescence sensor was constructed to measure the NADH-dependent fluorescence in Nicotiana tabacum plant cell culture and the analysis of fluorescence signal was done to be correlated with the viable cell weight. The structured kinetic model for cell growth was proposed to estimate the theoretical viable cell weight. The dimensional analysis was proposed for the interpretation of fluorescence signal, in which the path length, the inner filter effect and the hydrodynamic conditions were considered as the key factors on fluorescence signal. The dimensional analysis and empirical correlation of fluorescence signal to viable cell weight was applied to the interpretation of the detected fluorescence signal during cultivation. The proposed interpretation of fluorescence signal using dimensional analysis was well correlated with the viable cell weight estimated by the structured kinetic model as well as by empirical correlation.

引用

页码：528 / 534

页数：7

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