A SIMPLE AND RAPID PROCEDURE FOR THE DIRECT DETECTION OF CYTOMEGALOVIRUS IN URINE SAMPLES

被引:3
作者
LANDINI, MP [1 ]
TREVISANI, B [1 ]
GUAN, MX [1 ]
RIPALTI, A [1 ]
LAZZAROTTO, T [1 ]
LAPLACA, M [1 ]
机构
[1] IFCI CLONE SYST,CASALECCHIO RENO,ITALY
关键词
Cytomegalovirus; diagnosis; DNA probe; dot blot; ultrafiltration;
D O I
10.1002/jcla.1860040302
中图分类号
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号
1001 ;
摘要
We compared cytomegalovirus (CMV) isolation in tissue culture and viral DNA detection by DNA:DNA hybridization in 60 clinical urine samples concentrated by different procedures. Our results showed that urine concentration by filtration is the easiest and quickest procedure allowing further detection of CMV. With optimized working conditions, the CMV detection in urine can be done in 5 hr without requiring cell cultures or costly instruments. Copyright © 1990 Wiley Periodicals, Inc., A Wiley Company
引用
收藏
页码:161 / 164
页数:4
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