A PLANT HOMOLOG TO MAMMALIAN BRAIN 14-3-3 PROTEIN AND PROTEIN-KINASE-C INHIBITOR

被引：79

作者：

HIRSCH, S

AITKEN, A

BERTSCH, U

SOLL, J

机构：

[1] UNIV KIEL,INST BOT,OLSHAUSENSTR 40,W-2300 KIEL 1,GERMANY

[2] NATL INST MED RES,LONDON NW7 1AA,ENGLAND

[3] UNIV SAARBRUCKEN,FACHRICHTUNG,W-6600 SAARBRUCKEN,GERMANY

来源：

FEBS LETTERS | 1992年 / 296卷 / 02期

基金：

英国医学研究理事会;

关键词：

PROTEIN PHOSPHORYLATION; 14-3-3; PROTEIN; PROTEIN KINASE-C INHIBITOR;

D O I：

10.1016/0014-5793(92)80384-S

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have isolated cDNA clones of Spinacea oleracea L. and Oenothera hookeri of 930 and 1017 base pairs, respectively. The open reading frame deduced from the Oenothera sequence codes for a protein of a calculated molecular mass of 29 200. The primary amino acid sequence exhibits a very high degree (88%) of homology to the 14-3-3 protein from bovine brain, and protein kinase C inhibitor from sheep brain. Subsequently the plant protein was partially purified from leaf extract. The partially purified plant protein inhibited protein kinase C from sheep brain in a heterologous assay system. The active fraction consisted of 5-6 different polypeptides of similar molecular size. One of these proteins crossreacted with a peptide-specific antibody against protein kinase C inhibitor protein from sheep brain.

引用

页码：222 / 224

页数：3

共 14 条

[1]

AITKEN A, 1990, NATURE, V344, P594

[2]

ANSORGE W, 1982, ELECTROPHORESIS, V82, P235

[3] PURIFICATION, PROPERTIES, AND IMMUNOHISTOCHEMICAL LOCALIZATION OF HUMAN-BRAIN 14-3-3 PROTEIN [J].