A METHOD FOR QUICK DETERMINATION OF BILE-ACIDS IN BILE OF PATIENTS WITH BILIARY LITHIASIS

被引：4

作者：

COCA, E ^{[1
]}

RIBAS, B ^{[1
]}

TRIGUEROS, G ^{[1
]}

MTNEZSARMIENTO, J ^{[1
]}

BORQUE, M ^{[1
]}

ORTEGA, D ^{[1
]}

SOBRINO, A ^{[1
]}

MALLEN, A ^{[1
]}

DEPABLOS, I ^{[1
]}

FDEZREPRESA, JA ^{[1
]}

机构：

[1] UNIV MADRID,HOSP SAN CARLOS,DEPT CIRUGIA & APARATO DIGEST 1,MADRID 3,SPAIN

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1994年 / 17卷 / 06期

关键词：

D O I：

10.1080/10826079408013768

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A single high-performance liquid chromatographic method is described for determining and measuring the major conjugated bile acids in human bile. Four different chromatographic conditions were applied using c-18 columns and an isocratic solvent system. An effective one-step purification with Sep-pak C-18 was adopted. The advantages of this method are an improved separation of the conjugated bile acids within a short period of time. The detection limit was 0.0125 mu g/mu l and linearity was up to 6 Recovery was always up to 96.6 %, this being sufficient for routine clinical application during bile acids evaluation in biliary lithiasis.

引用

页码：1349 / 1363

页数：15

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