Abundance of Tylospora fibrillosa ectomycorrhizas in a South Swedish spruce forest measured by RFLP analysis of the PCR-amplified rDNA ITS region

被引:35
作者
Erland, S
机构
[1] Lund University, Lund, 223 62, Ecology Building
来源
MYCOLOGICAL RESEARCH | 1995年 / 99卷
关键词
D O I
10.1016/S0953-7562(09)80788-0
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Polymerase chain reaction (PCR) amplification of the internal transcribed spacer (ITS) region of the ribosomal DNA, subsequent cutting with the endonuclease Hinf I and restriction fragment length polymorphism (RFLP) analysis have previously proved to be a good method for distinguishing the ectomycorrhizal Tylospora fibrillosa from a large number of other basidiomycetes. In the present study this method was used to screen single mycorrhizas sampled in a Norway spruce forest in South Sweden. In this forest 98% of randomly collected mycorrhizas had the macroscopical features of T. fibrillosa mycorrhizas. Fungal ITS was successfully amplified from 93% of the sampled mycorrhizas. Five major, distinct fungal RFLP patterns were found, clearly demonstrating the benefit of the molecular method. The T. fibrillosa RFLP pattern was present in 21%, type 1 in 27%, type 2 in 20%, type 3 in 11% and type 4 in 3% of the mycorrhizas. Fungal ITS of more than one origin was found in 22% of the amplifications. It can be concluded that T. fibrillosa is one of the main mycorrhizal fungi in the studied site and it is therefore likely to be of significant ecological importance. These results suggest that T. fibrillosa would be a suitable 'model mycorrhiza' for studies of anthropogenic influence, such as pollution, liming or forest fertilization, on population structure of spruce mycorrhizas in southern Sweden.
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页码:1425 / 1428
页数:4
相关论文
共 13 条
[1]   EFFECTS OF TEMPERATURE AND INCUBATION-TIME ON THE ABILITY OF 3 ECTOMYCORRHIZAL FUNGI TO COLONIZE PINUS-SYLVESTRIS ROOTS [J].
ERLAND, S ;
FINLAY, R .
MYCOLOGICAL RESEARCH, 1992, 96 :270-272
[2]   IDENTIFICATION OF THE ECTOMYCORRHIZAL BASIDIOMYCETE TYLOSPORA-FIBRILLOSA DONK BY RFLP ANALYSIS OF THE PCR-AMPLIFIED ITS AND IGS REGIONS OF RIBOSOMAL DNA [J].
ERLAND, S ;
HENRION, B ;
MARTIN, F ;
GLOVER, LA ;
ALEXANDER, IJ .
NEW PHYTOLOGIST, 1994, 126 (03) :525-532
[3]  
ERLAND S, 1994, MYCOLOGICAL RES, V126, P525
[4]   IDENTIFICATION OF INDIGENOUS AND INTRODUCED SYMBIOTIC FUNGI IN ECTOMYCORRHIZAE BY AMPLIFICATION OF NUCLEAR AND MITOCHONDRIAL RIBOSOMAL DNA [J].
GARDES, M ;
WHITE, TJ ;
FORTIN, JA ;
BRUNS, TD ;
TAYLOR, JW .
CANADIAN JOURNAL OF BOTANY-REVUE CANADIENNE DE BOTANIQUE, 1991, 69 (01) :180-190
[5]   RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISMS IN THE NUCLEAR RIBOSOMAL DNA OF 4 LACCARIA-SPP - LABEO-BICOLOR, L-LACCATA, L-PROXIMA, AND L-AMETHYSTINA [J].
GARDES, M ;
FORTIN, JA ;
MUELLER, GM ;
KROPP, BR .
PHYTOPATHOLOGY, 1990, 80 (12) :1312-1317
[6]   ITS PRIMERS WITH ENHANCED SPECIFICITY FOR BASIDIOMYCETES - APPLICATION TO THE IDENTIFICATION OF MYCORRHIZAE AND RUSTS [J].
GARDES, M ;
BRUNS, TD .
MOLECULAR ECOLOGY, 1993, 2 (02) :113-118
[7]   RAPID IDENTIFICATION OF GENETIC-VARIATION OF ECTOMYCORRHIZAL FUNGI BY AMPLIFICATION OF RIBOSOMAL-RNA GENES [J].
HENRION, B ;
LETACON, F ;
MARTIN, F .
NEW PHYTOLOGIST, 1992, 122 (02) :289-298
[8]   TYPING TRUFFLE SPECIES BY PCR AMPLIFICATION OF THE RIBOSOMAL DNA SPACERS [J].
HENRION, B ;
CHEVALIER, G ;
MARTIN, F .
MYCOLOGICAL RESEARCH, 1994, 98 :37-43
[9]   RAPID PREPARATION OF DNA FROM FILAMENTOUS FUNGI [J].
RAEDER, U ;
BRODA, P .
LETTERS IN APPLIED MICROBIOLOGY, 1985, 1 (01) :17-20
[10]  
Sambrook J., 1989, MOL CLONING LAB MANU