PROLONGED CELL-FREE PROTEIN-SYNTHESIS IN A BATCH SYSTEM USING WHEAT-GERM EXTRACT

被引：27

作者：

KAWARASAKI, Y ^{[1
]}

NAKANO, H ^{[1
]}

YAMANE, T ^{[1
]}

机构：

[1] NAGOYA UNIV, SCH AGR,DEPT APPL BIOL SCI,MOLEC BIOTECHNOL LAB, CHIKUSA KU, NAGOYA, AICHI 46401, JAPAN

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1994年 / 58卷 / 10期

关键词：

D O I：

10.1271/bbb.58.1911

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Reaction conditions of cell-free protein synthesis using wheat germ extract were examined to prolong the period of protein synthesis in a batch reaction. By optimizing conditions for ATP regeneration system involved in the cell-free system, protein synthesis continued about 4 hours, so that about 17 mu g dihydrofolate reductase protein was obtained in 1 ml of a reaction mixture. It suggests that maintaining ATP concentration is the primary requirement for long-life cell-free protein synthesis.

引用

页码：1911 / 1913

页数：3

共 13 条

[1] ANDERSON CW, 1983, METHOD ENZYMOL, V101, P635
[2] PURIFICATION AND PROPERTIES OF ESCHERICHIA-COLI DIHYDROFOLATE-REDUCTASE
BACCANARI, D
PHILLIPS, A
SMITH, S
SINSKI, D
BURCHALL, J
[J]. BIOCHEMISTRY, 1975, 14 (24) : 5267 - 5273
[3] PRODUCTION OF AN ENZYMATIC ACTIVE PROTEIN USING A CONTINUOUS-FLOW CELL-FREE TRANSLATION SYSTEM
ENDO, Y
OTSUZUKI, S
ITO, K
MIURA, K
[J]. JOURNAL OF BIOTECHNOLOGY, 1992, 25 (03) : 221 - 230
[4] JOYCE BK, 1960, J BIOL CHEM, V235, P2278
[5] A CONTINUOUS CELL-FREE PROTEIN-SYNTHESIS SYSTEM FOR COUPLED TRANSCRIPTION-TRANSLATION
KIGAWA, T
YOKOYAMA, S
[J]. JOURNAL OF BIOCHEMISTRY, 1991, 110 (02) : 166 - 168
[6] HIGH-EFFICIENCY CELL-FREE SYNTHESIS OF PROTEINS - REFINEMENT OF THE COUPLED TRANSCRIPTION TRANSLATION SYSTEM
KUDLICKI, W
KRAMER, G
HARDESTY, B
[J]. ANALYTICAL BIOCHEMISTRY, 1992, 206 (02) : 389 - 393
[7] EXPRESSION OF SYNTHETIC SUPPRESSOR TRANSFER-RNA GENES UNDER THE CONTROL OF A SYNTHETIC PROMOTER
MASSON, JM
MILLER, JH
[J]. GENE, 1986, 47 (2-3) : 179 - 183
[8] AN INCREASED RATE OF CELL-FREE PROTEIN-SYNTHESIS BY CONDENSING WHEAT-GERM EXTRACT WITH ULTRAFILTRATION MEMBRANES
NAKANO, H
TANAKA, T
KAWARASAKI, Y
YAMANE, T
[J]. BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1994, 58 (04) : 631 - 634
[9] DIHYDROFOLATE REDUCTASE - PURIFICATION AND CHARACTERIZATION OF ENZYME FROM AN AMETHOPTERIN-RESISTANT MUTANT OF ESCHERICHIA-COLI
POE, M
HIRSHFIELD, JM
HOOGSTEEN, K
GREENFIELD, NJ
WILLIAMS, MN
[J]. BIOCHEMISTRY, 1972, 11 (06) : 1023 - +
[10] RYABOVA LA, 1988, NUCLEIC ACIDS RES, V17, P4412

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