A POLYMERASE CHAIN REACTION-BASED METHOD FOR THE DETECTION OF TRISOMY-12 IN B-CHRONIC LYMPHOCYTIC-LEUKEMIA

被引：6

作者：

BAKER, BW

HEIGHWAY, J

BROWETT, PJ

NORTON, JD

机构：

[1] CHRISTIE HOSP & HOLT RADIUM INST,NHS TRUST,PATERSON INST CANC RES,CRC DEPT GENE REGULAT,MANCHESTER M20 9BX,LANCS,ENGLAND

[2] UNIV AUCKLAND,DEPT MOLEC MED,AUCKLAND,NEW ZEALAND

[3] CHRISTIE HOSP & HOLT RADIUM INST,NHS TRUST,PATERSON INST CANC RES,CRC DEPT CANC GENET,MANCHESTER M20 9BX,LANCS,ENGLAND

来源：

BRITISH JOURNAL OF HAEMATOLOGY | 1993年 / 85卷 / 01期

关键词：

D O I：

10.1111/j.1365-2141.1993.tb08664.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

We report a polymerase chain reaction (PCR)-based method for detection of clonal trisomy 12 cell populations in B cell chronic lymphocytic leukaemia which is particulary applicable to retrospective analysis or archival material. The method utilizes three restriction enzyme site polymorphisms linked to the KRAS2 locus on chromosome 12 to monitor allele imbalance resulting from duplication of this chromosome. 21/24 unselected chronic lymphocytic leukaemia cases (88%) were informative for at least one polymorphism and of these, eight (33% of total cases) displayed allelic imbalance consistent with trisomy 12.

引用

页码：179 / 181

页数：3

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