CAATTG-SPECIFIC RESTRICTION-MODIFICATION MUNI GENES FROM MYCOPLASMA - SEQUENCE SIMILARITIES BETWEEN R-CENTER-DOT-MUNI AND R-CENTER-DOT-ECORI

被引：29

作者：

SIKSNYS, V ^{[1
]}

ZARECKAJA, N ^{[1
]}

VAISVILA, R ^{[1
]}

TIMINSKAS, A ^{[1
]}

STAKENAS, P ^{[1
]}

BUTKUS, V ^{[1
]}

JANULAITIS, A ^{[1
]}

机构：

[1] FERMENTAS, INST BIOTECHNOL, VILNIUS 2028, LITHUANIA

来源：

GENE | 1994年 / 142卷 / 01期

关键词：

RECOMBINANT DNA; PROTEIN-DNA INTERACTIONS; SEQUENCE SIMILARITIES; ENDONUCLEASE; METHYLTRANSFERASE; CLONING;

D O I：

10.1016/0378-1119(94)90347-6

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The genes coding for the MunI restriction-modification (R-M) system, which recognize the sequence 5'-CAATTG, have been cloned and expressed in Escherichia coli, and their nucleotide sequences have been determined. The restriction endonuclease (ENase; R MunI) is encoded by an open reading frame (ORF) of 606 bp, and a 699-bp ORF codes for the methyltransferase (MTase). The two genes are transcribed divergently from a 355-bp region. The gene encoding the ENase is preceded by a short co-linear ORF of 222 bp. The deduced amino acid (aa) sequence of this short ORF (SORF) closely resembles the sequences of a family of regulatory proteins that are associated with other type-II R-M systems. Comparative analysis of the deduced aa sequence of R.MunI revealed several regions of similarity to the EcoRI and RsrI ENases that recognize the GAATTC sequence. The similar mode of interaction of MunI, EcoRI and Rsr I with the tetranucleotide AATT, common to the recognition sequences of these ENases, was suggested.

引用

页码：1 / 8

页数：8

共 40 条

[1]

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