INVITRO SYNTHESIS OF 16-ALPHA-HYDROXYESTRONE BY FEMALE RAT-LIVER MICROSOMES - ITS POSSIBLE ROLE IN THE ETIOLOGY OF BREAST-CANCER

被引：4

作者：

ARTS, CJM ^{[1
]}

WILMER, JWGM ^{[1
]}

DEBIE, ATHJ ^{[1
]}

VANDENBERG, H ^{[1
]}

机构：

[1] TNO,CIVO,INST TOXICOL & NUTR,DEPT BIOL TOXICOL,3700 AJ ZEIST,NETHERLANDS

来源：

JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY | 1990年 / 36卷 / 06期

关键词：

D O I：

10.1016/0022-4731(90)90169-S

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Liver homogenates from female rat strains (Sprague-Dawley, Wistar and Fisher) were incubated in a NADPH regenerating medium in the presence of labelled and unlabelled estrone. Liver microsomes isolated from male rats and female mice were used as positive controls. Using HPLC and paper chromatography, under the experimental conditions used it was found that liver homogenates from female rats were able to convert estrone to various metabolites such as 16α-hydroxyestrone. In a mutagenicity assay (Ames test), with 16α-hydroxyestrone as test substance, two strains (TA98 and TA 1538) of the five strains tested showed a 2-3-fold increase in the number of his+ revenants relative to the control values. Estrone did not cause any mutagens in the test used. It is concluded that female rats are able to synthesize 16α-hydroxyestrone in vitro. Whether this compound is risk factor for breast cancer remains unclear. © 1990.

引用

页码：527 / 531

页数：5

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