KINETIC AND STEREOELECTRONIC EFFECTS OF A FLUORINE SUBSTITUENT ON THE REACTION CATALYZED BY AN NADPH-DEPENDENT CYCLOHEX-1-ENYLCARBONYL COA REDUCTASE

被引：7

作者：

BRIDGE, CF ^{[1
]}

OHAGAN, D ^{[1
]}

REYNOLDS, KA ^{[1
]}

WALLACE, KK ^{[1
]}

机构：

[1] UNIV MARYLAND,SCH PHARM,DEPT PHARMACEUT SCI,BALTIMORE,MD 21201

来源：

JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS | 1995年 / 22期

关键词：

D O I：

10.1039/c39950002329

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The introduction of a fluorine atom at C-3 of cyclohex-1-enecarbonyl CoA has a dramatic effect on the processing of the fluorinated over the natural analogue by NADPH dependant cyclohex-1-enecarbonyl CoA reductase from Streptomyces collinus, the fluorinated analogue is processed with a five fold increase in V-max and kinetic isotope studies suggest that hydride delivery is only partially rate limiting in the latter case; the enzyme also shows a small kinetic preference-for axial over equatorial fluorine at C-3 of 3-fluorocyclohex-1-encarbonyl CoA 4.

引用

页码：2329 / 2330

页数：2