HUMAN THROMBOPOIETIN - GENE STRUCTURE, CDNA SEQUENCE, EXPRESSION, AND CHROMOSOMAL LOCALIZATION

被引：130

作者：

FOSTER, DC ^{[1
]}

SPRECHER, CA ^{[1
]}

GRANT, FJ ^{[1
]}

KRAMER, JM ^{[1
]}

KUIJPER, JL ^{[1
]}

HOLLY, RD ^{[1
]}

WHITMORE, TE ^{[1
]}

HEIPEL, MD ^{[1
]}

BELL, LA ^{[1
]}

CHING, AFT ^{[1
]}

MCGRANE, V ^{[1
]}

HART, C ^{[1
]}

OHARA, PJ ^{[1
]}

LOK, S ^{[1
]}

机构：

[1] ZYMOGENET INC,SEATTLE,WA 98102

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1994年 / 91卷 / 26期

关键词：

D O I：

10.1073/pnas.91.26.13023

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Thrombopoietin (TPO), a lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from committed progenitor cells, is believed to be the major physiological regulator of circulating platelet levels. Recently we have isolated a cDNA encoding a ligand for the murine c-mpl protooncogene and shown it to be TPO. By employing a murine cDNA probe, we have isolated a gene encoding human TPO from a human genomic library. The TPO locus spans over 6 kb and has a structure similar to that of the erythropoietin gene (EPO). Southern blot analysis of human genomic DNA reveals a hybridization pattern consistent with a single gene locus. The locus was mapped by in situ hybridization of metaphase chromosome preparations to chromosome 3q26-27, a site where a number of chromosomal abnormalities associated with thrombocythemia in cases of acute myeloid leukemia have been mapped. A human TPO cDNA was isolated by PCR from kidney mRNA. The cDNA encodes a protein with 80% identity to previously described murine TPO and is capable of initiating a proliferative signal to murine interleukin 3-dependent BaF3 cells expressing the murine or human TPO receptor.

引用

页码：13023 / 13027

页数：5

共 47 条

[1] RETROVIRAL INSERTIONS 90 KILOBASES PROXIMAL TO THE EVI-1 MYELOID TRANSFORMING GENE ACTIVATE TRANSCRIPTION FROM THE NORMAL PROMOTER [J].