GENES FOR THE PLASTID ELONGATION-FACTOR TU AND RIBOSOMAL-PROTEIN S7 AND 6 TRANSFER-RNA GENES ON THE 73-KB DNA FROM ASTASIA-LONGA THAT RESEMBLES THE CHLOROPLAST DNA OF EUGLENA

被引：47

作者：

SIEMEISTER, G ^{[1
]}

BUCHHOLZ, C ^{[1
]}

HACHTEL, W ^{[1
]}

机构：

[1] UNIV BONN,INST BOT,W-5300 BONN,GERMANY

来源：

MOLECULAR & GENERAL GENETICS | 1990年 / 220卷 / 03期

关键词：

Astasia longa; ORFs; Plastid DNA; rps7; tuf and tRNA genes; Transcription;

D O I：

10.1007/BF00391749

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The nucleotide sequence of a 6156 by segment of the circular 73 kb DNA from Astasia longa resembling the chloroplast DNA of Euglena was determined. The genes for the plastid elongation factor Tu (tufA) and the ribosomal protein S7 (rps7), six tRNA genes (trnQ, trnS, trnG, trnM, trnT, trnR), and three open reading frames were identified. These genes show a high degree of sequence similarity (73%-99%) to the corresponding genes on the Euglena chloroplast genome. The tufA gene contains two small AT-rich introns within its coding region. Northern analysis revealed the in vivo transcription of the tufA gene and of a reading frame of 456 codons into monocistronic mRNAs of 1.3 and 1.4 kb, respectively. The arrangement and organization of the genes on the 73 kb DNA of the colourless heterotrophic flagellate Astasia and the chloroplast DNA of autotrophic Euglena are compared. © 1990 Springer-Verlag.

引用

页码：425 / 432

页数：8

共 30 条

[1] SUPERCOIL SEQUENCING - A FAST AND SIMPLE METHOD FOR SEQUENCING PLASMID DNA [J].