CHEMICAL NUCLEASE ACTIVITY OF 5-PHENYL-1,10-PHENANTHROLINE-COPPER ION DETECTS INTERMEDIATES IN TRANSCRIPTION INITIATION BY ESCHERICHIA-COLI RNA-POLYMERASE

被引：67

作者：

THEDERAHN, T

SPASSKY, A

KUWABARA, MD

SIGMAN, DS

机构：

[1] UNIV CALIF LOS ANGELES,SCH MED,DEPT BIOL CHEM,LOS ANGELES,CA 90024

[2] UNIV CALIF LOS ANGELES,INST MOLEC BIOL,LOS ANGELES,CA 90024

[3] INST PASTEUR,F-75724 PARIS 15,FRANCE

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 168卷 / 02期

关键词：

D O I：

10.1016/0006-291X(90)92386-E

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The nuclease activity of the copper complex of 5-phenyl-1,10-phenanthroline (5-φ-OP-CU) detects conformational changes in the lac UV-5 promoter caused by E. Coli RNA polymerase. The template strand in melted regions of initiation complexes upstream of the site of nucleotide triphosphate incorporation is very reactive. In open and elongation complexes, downstream scission sites (e. g. +4 and +5 for the open complex) on both strands are observed. The patterns of both downstream cutting sites suggest an atypical double helix at the leading edge of the transcription bubble. © 1990.

引用

页码：756 / 762

页数：7

共 15 条

[1] CLEAVAGE OF DNA WITH METHIDIUMPROPYL-EDTA-IRON(II) - REACTION CONDITIONS AND PRODUCT ANALYSES [J].