DODECA SATELLITE - A CONSERVED G+C-RICH SATELLITE FROM THE CENTROMERIC HETEROCHROMATIN OF DROSOPHILA-MELANOGASTER

被引：107

作者：

ABAD, JP

CARMENA, M

BAARS, S

SAUNDERS, RDC

GLOVER, DM

LUDENA, P

SENTIS, C

TYLERSMITH, C

VILLASANTE, A

机构：

[1] UNIV AUTONOMA MADRID,CSIC,CTR BIOL MOLEC,CANTOBLANCO,E-28049 MADRID,SPAIN

[2] UNIV AUTONOMA MADRID,CSIC,CTR NACL BIOTECNOL,E-28049 MADRID,SPAIN

[3] UNIV AUTONOMA MADRID,FAC CIENCIAS,DEPT BIOL,E-28049 MADRID,SPAIN

[4] UNIV DUNDEE,DEPT BIOCHEM,CANC RES CAMPAIGN,CELL CYCLE GENET GRP,DUNDEE DD1 4HN,SCOTLAND

[5] UNIV OXFORD,DEPT BIOCHEM,OXFORD OX1 3QU,ENGLAND

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1992年 / 89卷 / 10期

关键词：

CENTROMERIC SATELLITE; DROSOPHILA YEAST ARTIFICIAL CHROMOSOME LIBRARY; INSITU HYBRIDIZATION;

D O I：

10.1073/pnas.89.10.4663

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

To identify sequences from the centromeric region, we have constructed a Drosophila melanogaster yeast artificial chromosome (YAC) library and screened it with purified DNA from the minichromosome Dp(1;f)1187 derived from the X chromosome. We describe the structure of one clone isolated in this way. This YAC is structurally unstable and contains tandemly repeated G + C-rich 11-mer and 12-mer units, which we call dodeca satellite. Most of this satellite is located near the centromere of an autosome. Cross-hybridizing sequences are found in the genomes of organisms as distant as Arabidopsis thaliana and Homo sapiens.

引用

页码：4663 / 4667

页数：5

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