PROTEIN SECRETION BY THE MOUSE BLASTOCYST - STIMULATORY EFFECT ON SECRETION INTO THE BLASTOCOELE BY TRANSFORMING GROWTH FACTOR-ALPHA

被引：18

作者：

DARDIK, A ^{[1
]}

DOHERTY, AS ^{[1
]}

SCHULTZ, RM ^{[1
]}

机构：

[1] UNIV PENN,DEPT BIOL,PHILADELPHIA,PA 19104

来源：

MOLECULAR REPRODUCTION AND DEVELOPMENT | 1993年 / 34卷 / 04期

关键词：

TGF-ALPHA; GELATINASE; PROTEIN SECRETION;

D O I：

10.1002/mrd.1080340408

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have previously demonstrated that newly synthesized proteins are secreted into the mouse blastocoel [Dardik and Schultz (1991): Biol Reprod 45:328-333]. In the present study we examine the effect of transforming growth factor-alpha (TGF-alpha) on these proteins. We observe that TGF-alpha stimulates secretion of these newly synthesized proteins into the blastocoel and apical medium, which faces the zona pellucida, by about 65%. Although one-dimensional gel electrophoretic analysis does not reveal any marked differences in the patterns of newly synthesized proteins secreted into the blastocoel in response to TGF-alpha, zymography reveals a marked stimulation in the secretion of several gelatinases into the blastocoel and apical medium. These results suggest additional functions for TGF-alpha in mouse preimplantation development.

引用

页码：396 / 401

页数：6

共 25 条

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[2]

Alexander CM, 1991, CELL BIOL EXTRACELLU, P255

[3]

BEHRENDTSEN O, 1992, DEVELOPMENT, V114, P447

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