SELECTIVE CHEMILUMINESCENT DETERMINATION OF ADENOSINE-5'-TRIPHOSPHATE BY A FLOW-INJECTION METHOD

A new chemiluminescence reaction system using hydrogen peroxide/sodium hydroxide/brilliant sulfoflavine was successfully applied to a flow-injection analysis (FIA) for the specific determination of adenosine-5'-triphosphate (ATP). The analytical system comprised two flow lines (L1 and L2) with a 20 mul injector; 5% hydrogen peroxide and 5.0X10(-3) M (Mol/1) sodium hydroxide/ 1.0X10(-3) M brilliant sulfoflavine were passed through the L1 and L2 at a flow rate of 4.5 ml/min, respectively. The analytical properties of ATP were as follows: the lower detection limit was 1.0X10(-5) M (S/N=2); the determination range, 1.0x10(-5)-5.0X10(-3) M; the analytical time required, 8 s; the reproducibility, 2.1% in terms of the relative standard deviation in 5 repeated runs for 5X10(-3) M ATP. Regarding the selectivity, the CL intensity for 1.0X10(-3) M ATP was not interfered with by other nucleotides coexisting at 100-times higher concentration than ATP. A recovery test of ATP in a human urine matrix was also performed.