DNA/RNA SYNTHESIS AND LABELING

被引：12

作者：

GAIT, MJ ^{[1
]}

机构：

[1] MRC, MOLEC BIOL LAB, CAMBRIDGE, ENGLAND

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 1991年 / 2卷 / 01期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1016/0958-1669(91)90062-A

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Reliable methods of machine-aided RNA synthesis have been established to complement those for DNA assembly. Oligonucleotides containing thio-modified backbones and 2'-O-alkyl sugars head the list of many newly available analogues. Biotin, fluorescent agents and many reporter groups can be conveniently introduced into oligonucleotides in multiples by phosphoramidite or H-phosphonate chemistry.

引用

收藏

页码：61 / 68

页数：8

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