FIBER-OPTIC-BASED IMMUNOSENSORS FOR HAPTENS

被引：27

作者：

BETTS, TA

CATENA, GC

HUANG, JF

LITWILER, KS

ZHANG, J

ZAGROBELNY, J

BRIGHT, FV

机构：

[1] Department of Chemistry, State University of New York at Buffalo, Buffalo, NY 14214, Acheson Hall

来源：

ANALYTICA CHIMICA ACTA | 1991年 / 246卷 / 01期

基金：

美国国家科学基金会;

关键词：

FLUOROMETRY; FIBEROPTIC SENSOR; IMMUNOSENSORS; HAPTENS;

D O I：

10.1016/S0003-2670(00)80664-9

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A selective fiber-optic-based immunosensor for haptens is described and characterized. In order to construct a sensor for different analytes, a prescribed reaction protocol is followed and only the recognition element [F(ab') antibody fragment] has to be changed. The dynamic range of this sensor is about two orders of magnitude and the detection limits are at least 25 nM (25-mu-l volume). By using steady-state fluorescence and classical ultra-high-vacuum surface analytical techniques, the effects of regeneration media, reaction and storage times and interferents on the sensor response were studied.

引用

页码：55 / 63

页数：9

共 24 条

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