CONCENTRATIONS OF IMMUNOREACTIVE INTERLEUKIN-1 AND INTERLEUKIN-2 IN HUMAN PREOVULATORY FOLLICULAR-FLUID

被引：89

作者：

WANG, LJ ^{[1
]}

NORMAN, RJ ^{[1
]}

机构：

[1] UNIV ADELAIDE,QUEEN ELIZABETH HOSP,DEPT OBSTET & GYNAECOL,WOODVILLE,SA 5011,AUSTRALIA

来源：

HUMAN REPRODUCTION | 1992年 / 7卷 / 02期

关键词：

INTERLEUKINS; IL-1; IL-2; FOLLICULAR FLUID; IMMUNOASSAY;

D O I：

10.1093/oxfordjournals.humrep.a137607

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

The presence of immunoreactive interleukin-1 (IL-1) and interleukin-2 (IL-2) in human follicular fluid obtained at the time of oocyte collection for in-vitro fertilization was ascertained by radioimmunoassay. In group I (20 fluids from 20 patients), the concentrations of IL-1 were 0.9 +/- 0.06 and 1.9 +/- 0.04 (mean +/- SEM) fmol/l in follicular fluid and plasma respectively. A positive correlation existed between IL-1 levels in follicular fluid and plasma (r = 0.56, P < 0.01). Concentrations of IL-2 were 3.5 +/- 0.2 and 6.1 +/- 0.3 fmol/l in follicular fluid and plasma respectively. A positive correlation of IL-2 levels was also found between follicular fluid and plasma (r = 0.65, P < 0.01). There was no association between IL-1, IL-2 and steroid levels, regardless of whether they were compared in follicular fluid or plasma. Group II was composed of a series of fluids (two to seven samples for each of seven patients) in which the follicular concentrations of IL-1 and IL-2 did not show a positive correlation with the volume of follicular fluid or the concentrations of follicular fluid steroids. It is concluded that human preovulatory follicular fluid contains immunoreactive IL-1 and IL-2. The role of IL-1 and IL-2 in ovarian physiology remains to be determined.

引用

页码：147 / 150

页数：4

共 20 条

[1]

Bomsel-Helmreich O., Gougeon A., Thebault A., Saltarelli D., Milgrom E., Frydman R., Papiemik E., Healthy and atretic human follicles in the preovulatory phase: Differences in evolution of follicular morphology and steroid content of follicular fluid, J. Clin. Endocrinol. Metab, 48, pp. 686-694, (1979)

[2]

Carson R.S., Trounson A.O., Findlay J.K., Successful fertilisation of human oocytes in vitro: Concentration of estradiol-17/3, progesterone and and rostenedione in the antral fluid of donor follicles, J. Clin. Endocrinol. Metab, 55, pp. 798-800, (1982)

[3]

Durum S.K., Schmidt J.A., Oppenheim J.J., Interleukin-1: An immunological perspective, Annu. Rev. Immunol, 3, pp. 263-287, (1985)

[4]

Fukuoka M., Mori T., Taii S., Yasuda K., Interleukin-1 inhibits luteinization of porcine granulosa cells in culture, Endocrinology, 122, pp. 367-369, (1988)

[5]

Gorospe W.C., Kasson B.G., Lymphokines from concana-valin-A-stimulated lymphocytes regulate rat granulosa cell steroidogenesis in vitro, Endocrinology, 123, pp. 2462-2471, (1988)

[6]

Gottschall P.E., Uehara A., Hoffmann S.T., Arimura A., Interleukin-1 inhibits follicle-stimulating hormone-induced differentiation in rat granulosa cells in vitro, Biochem. Biophys. Res. Common, 149, pp. 502-509, (1987)

[7]

Hagiwara H., Huang H.S., Arai N., Herzenberg L.A., Arai K.-I., Zlotnik A., Interleukin-1 modulates messenger RNA levels of lymphokines and of other molecules associated with T cell activation in the T cell lymphoma LBRM33-LA5, J. Immunol, 138, pp. 2514-2519, (1987)

[8]

Kasson B.G., Gorospe W.C., Effects of interleukin-1, 2 and 3 on follicle-stimulating hormone-induced differentiation of rat granulosa cells, Mol. Cell. Endocrinol, 62, pp. 103-114, (1989)

[9]

Kerin J.F., Wames G.M., Quinn P., Kirby C.A., Jeffrey R., Matthews C.D., Seamark R.F., Cox L.W., In vitro fertilisation and embryo transfer program. The University of Adelaide. J, Vitro Fertil. Embryo Transfer, 1, pp. 63-71, (1984)

[10]

Lei Z.M., Chegini N., Rao C., Quantitative cell composition of human and bovine corpora lutea from various reproductive states, Biol. Reprod, 44, pp. 1148-1156, (1991)

← 1 2 →