INTRACELLULAR IMMUNIZATION OF HUMAN FETAL CORD-BLOOD STEM PROGENITOR CELLS WITH A RIBOZYME AGAINST HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1

被引：111

作者：

YU, M

LEAVITT, MC

MARUYAMA, M

YAMADA, O

YOUNG, D

HO, AD

WONGSTAAL, F

机构：

[1] UNIV CALIF SAN DIEGO,DEPT MED,LA JOLLA,CA 92093

[2] UNIV CALIF SAN DIEGO,DEPT BIOL,LA JOLLA,CA 92093

[3] UNIV CALIF SAN DIEGO,STEM CELL TRANSPLANTAT PROGRAM,SAN DIEGO,CA 92093

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1995年 / 92卷 / 03期

关键词：

D O I：

10.1073/pnas.92.3.699

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Successful treatment of human immunodeficiency virus infection may ultimately require targeting of hematopoietic stem cells. Here we used retroviral vectors carrying the ribozyme gene to transduce CD34(+) cells from human fetal cord blood. Transduction and ribozyme expression had no apparent adverse effect on cell differentiation and/or proliferation. The macrophage-like cells, differentiated from the stem/progenitor cells in vitro, expressed the ribozyme gene and resisted infection by a macrophage tropic human immunodeficiency virus type 1. These results suggest the feasibility of stem cell gene therapy for human immunodeficiency virus-infected patients.

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页码：699 / 703

页数：5

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