GAS-PHASE INFRARED-SPECTRA OF 209-POLYCHLORINATED BIPHENYL CONGENERS USING GAS-CHROMATOGRAPHY WITH FOURIER-TRANSFORM INFRARED DETECTION - INTERNAL STANDARDIZATION WITH A C-13-LABELED CONGENER

The gas phase infrared spectra of biphenyl and 209 polychlorinated biphenyls (PCB) are observed; 30 are presented in graphical form so that their uniqueness can be appreciated. The spectra were observed with a gas chromatograph linked via a light pipe with a Fourier transform infrared spectrometer. Unequivocal library matches (with match factors > 990 in 1000) can be performed with 50 ng of a congener injected into the chromatograph. Quantitation with the technique was briefly investigated with 2,2',5,5'-tetrachlorobiphenyl (BZ no. 52). Selective wavelength chromatograms of the most intense absorption (1457cm(-1)) were integrated. Septuplicate injections showed that 20 ng of the compound could be determined with +/-4% precision. The resolution of the spectrometer was sufficient to distinguish between the C-13-labelled trichlorobiphenyl and the unlabelled material, indicating the possibility of internal or surrogate standardization of PCB analysis.