ENHANCING THE THERMOSTABILITY OF GLUCOSE-ISOMERASE BY PROTEIN ENGINEERING

被引：45

作者：

QUAX, WJ ^{[1
]}

MRABET, NT ^{[1
]}

LUITEN, RGM ^{[1
]}

SCHUURHUIZEN, PW ^{[1
]}

STANSSENS, P ^{[1
]}

LASTERS, I ^{[1
]}

机构：

[1] PLANT GENET SYST NV,DEPT PROT ENGN,B-9000 GHENT,BELGIUM

来源：

BIO-TECHNOLOGY | 1991年 / 9卷 / 08期

关键词：

D O I：

10.1038/nbt0891-738

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have engineered recombinant glucose isomerase (GI) from Actinoplanes missouriensis by site-directed mutagenesis to enhance its thermal stability in both the soluble and immobilized forms. Substitution of arginine for lysine at position 253, which lies at the dimer/dimer interface of the GI tetramer, produced the largest stabilization under model industrial conditions. We discuss our results in terms of a model in which chemical glycation of lysines by sugars in the industrial corn syrup substrate represents a major pathway of destabilization.

引用

页码：738 / 742

页数：5

共 30 条

[1]

ALBER T, 1989, ANNU REV BIOCHEM, V58, P765, DOI 10.1146/annurev.biochem.58.1.765

[2] TEMPERATURE-SENSITIVE MUTATIONS OF BACTERIOPHAGE-T4 LYSOZYME OCCUR AT SITES WITH LOW MOBILITY AND LOW SOLVENT ACCESSIBILITY IN THE FOLDED PROTEIN [J].

ALBER, T ;

SUN, DP ;

NYE, JA ;

MUCHMORE, DC ;

MATTHEWS, BW .

BIOCHEMISTRY, 1987, 26 (13) :3754-3758

[3] XYLOSE ISOMERASE FROM ACTINOPLANES-MISSOURIENSIS - PRIMARY STRUCTURE OF THE GENE AND THE PROTEIN [J].

AMORE, R ;

HOLLENBERG, CP .

NUCLEIC ACIDS RESEARCH, 1989, 17 (18) :7515-7515

[4] FURTHER IDENTIFICATION OF NATURE AND LINKAGE OF CARBOHYDRATE IN HEMOGLOBIN-A1C [J].

BUNN, HF ;

HANEY, DN ;

GABBAY, KH ;

GALLOP, PM .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1975, 67 (01) :103-109

[5] CATALYTIC PROPERTIES OF D-XYLOSE ISOMERASE FROM STREPTOMYCES-VIOLACEORUBER [J].

CALLENS, M ;

KERSTERSHILDERSON, H ;

VANOPSTAL, O ;

DEBRUYNE, CK .

ENZYME AND MICROBIAL TECHNOLOGY, 1986, 8 (11) :696-700

[6] X-RAY-ANALYSIS OF D-XYLOSE ISOMERASE AT 1.9 A - NATIVE ENZYME IN COMPLEX WITH SUBSTRATE AND WITH A MECHANISM-DESIGNED INACTIVATOR [J].

CARRELL, HL ;

GLUSKER, JP ;

BURGER, V ;

MANFRE, F ;

TRITSCH, D ;

BIELLMANN, JF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (12) :4440-4444

[7]

CHEN WP, 1980, PROCESS BIOCHEM, P36

[8]

CHEN WP, 1980, PROCESS BIOCHEM, P30

[9]

COLSON C, 1965, GENETICS, V52, P1043

[10] THE 3.0 A CRYSTAL-STRUCTURE OF XYLOSE ISOMERASE FROM STREPTOMYCES-OLIVOCHROMOGENES [J].

FARBER, GK ;

PETSKO, GA ;

RINGE, D .

PROTEIN ENGINEERING, 1987, 1 (06) :459-466

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