PURIFICATION AND PARTIAL AMINO-ACID-SEQUENCE OF CURVATICIN FS47, A HEAT-STABLE BACTERIOCIN PRODUCED BY LACTOBACILLUS-CURVATUS FS47

被引：55

作者：

GARVER, KI ^{[1
]}

MURIANA, PM ^{[1
]}

机构：

[1] PURDUE UNIV, DEPT FOOD SCI, W LAFAYETTE, IN 47907 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1994年 / 60卷 / 06期

关键词：

D O I：

10.1128/AEM.60.6.2191-2195.1994

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Curvaticin FS47, a bacteriocin produced by Lactobacillus curvatus FS47, is inhibitory to Listeria monocytogenes, as well as Lactobacillus, Pediococcus, Enterococcus, and Bacillus spp. The bacteriocin was purified by 40% ammonium sulfate precipitation, solid-phase extraction, and reversed-phase high-pressure liquid chromatography. Purified curvaticin FS47 was determined to be 4.07 kDa by mass spectrometry and was partially sequenced. Thirty-one N-terminal amino acids were identified; the curvaticin FS47 protein sequence did not show homology to the pediocin-like group of bacteriocins.

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页码：2191 / 2195

页数：5

相关论文

共 38 条

[1] BASIC LOCAL ALIGNMENT SEARCH TOOL [J].

ALTSCHUL, SF ;

GISH, W ;

MILLER, W ;

MYERS, EW ;

LIPMAN, DJ .

JOURNAL OF MOLECULAR BIOLOGY, 1990, 215 (03) :403-410

[2] A HIGHLY EFFICIENT 2ND-STEP CONCENTRATION TECHNIQUE FOR BACTERIOPHAGES AND ENTERIC VIRUSES USING AMMONIUM-SULFATE AND TWEEN-80 [J].

ARMON, R ;

ARELLA, M ;

PAYMENT, P .

CANADIAN JOURNAL OF MICROBIOLOGY, 1988, 34 (05) :651-655

[3] DETECTION AND ACTIVITY OF LACTACIN-B, A BACTERIOCIN PRODUCED BY LACTOBACILLUS-ACIDOPHILUS [J].

BAREFOOT, SF ;

KLAENHAMMER, TR .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1983, 45 (06) :1808-1815

[4] CHANNEL-FORMING PROPERTIES OF CECROPINS AND RELATED MODEL COMPOUNDS INCORPORATED INTO PLANAR LIPID-MEMBRANES [J].

CHRISTENSEN, B ;

FINK, J ;

MERRIFIELD, RB ;

MAUZERALL, D .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (14) :5072-5076

[5]

FURTH AJ, 1984, METHOD ENZYMOL, V104, P318

[6] DETECTION, IDENTIFICATION AND CHARACTERIZATION OF BACTERIOCIN-PRODUCING LACTIC-ACID BACTERIA FROM RETAIL FOOD-PRODUCTS [J].

GARVER, KI ;

MURIANA, PM .

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 1993, 19 (04) :241-258

[7] CHARACTERIZATION OF LEUCOCIN-A-UAL-187 AND CLONING OF THE BACTERIOCIN GENE FROM LEUCONOSTOC-GELIDUM [J].

HASTINGS, JW ;

SAILER, M ;

JOHNSON, K ;

ROY, KL ;

VEDERAS, JC ;

STILES, ME .

JOURNAL OF BACTERIOLOGY, 1991, 173 (23) :7491-7500

[8] CHARACTERIZATION AND PURIFICATION OF MESENTERICIN Y105, AN ANTI-LISTERIA BACTERIOCIN FROM LEUCONOSTOC-MESENTEROIDES [J].

HECHARD, Y ;

DERIJARD, B ;

LETELLIER, F ;

CENATIEMPO, Y .

JOURNAL OF GENERAL MICROBIOLOGY, 1992, 138 :2725-2731

[9] PURIFICATION AND PRIMARY STRUCTURE OF PEDIOCIN PA-1 PRODUCED BY PEDIOCOCCUS-ACIDILACTICI PAC-10 [J].

HENDERSON, JT ;

CHOPKO, AL ;

VANWASSENAAR, PD .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1992, 295 (01) :5-12

[10] INFLUENCE OF FAT AND EMULSIFIERS ON THE EFFICACY OF NISIN IN INHIBITING LISTERIA-MONOCYTOGENES IN FLUID MILK [J].

JUNG, DS ;

BODYFELT, FW ;

DAESCHEL, MA .

JOURNAL OF DAIRY SCIENCE, 1992, 75 (02) :387-393

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