INTERLEUKIN-6 PRODUCTION BY U373 MG, A HUMAN ASTROCYTOMA CELL-LINE - DIFFERENT PATHWAYS INVOLVED IN SUBSTANCE-P AND LIPOPOLYSACCHARIDE ACTIVATION

被引：24

作者：

PALMA, C ^{[1
]}

URBANI, F ^{[1
]}

MANZINI, S ^{[1
]}

机构：

[1] IST SUPER SANITA,BACTERIOL & MED MYCOL LAB,I-00161 ROME,ITALY

来源：

JOURNAL OF NEUROIMMUNOLOGY | 1995年 / 59卷 / 1-2期

关键词：

ASTROCYTOMA; INTERLEUKIN-6; SUBSTANCE P; LIPOPOLYSACCHARIDE; STAUROSPORINE; CALMODULIN ANTAGONIST;

D O I：

10.1016/0165-5728(95)00040-9

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Substance P (SP) and lipopolysaccharide (LPS) stimulated interleukin-6 (IL-6) gene expression, as well as IL-6 protein secretion in the human astrocytoma cell line U373 MG. Staurosporine, an inhibitor of protein kinase C (PKC), entirely blocked SP- but not LPS-induced IL-6 release. In addition, the down regulation of PKC inhibited the SP response and only marginally altered LPS activation. Differently from SP, LPS-induced IL-6 release was markedly reduced by W7, a calmodulin antagonist. Moreover, SP interacted in a synergistic manner with LPS. Thus, neural (SP) and bacterial (LPS) mediators stimulate U373 MG IL-6 release via distinct, though not antagonistic, activation pathways.

引用

页码：155 / 163

页数：9

共 49 条

[1]

BARBER RD, 1979, J COMP NEUROL, V198, P331

[2] IL-6-MEDIATED EVENTS IN ALZHEIMERS-DISEASE PATHOLOGY [J].

BAUER, J ;

STRAUSS, S ;

VOLK, B ;

BERGER, M .

IMMUNOLOGY TODAY, 1991, 12 (11) :422-422

[3]

BEYAERT R, 1993, CANCER RES, V53, P2623

[4] SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION [J].

CHOMCZYNSKI, P ;

SACCHI, N .

ANALYTICAL BIOCHEMISTRY, 1987, 162 (01) :156-159

[5] MOLECULAR PROFILE OF REACTIVE ASTROCYTES - IMPLICATIONS FOR THEIR ROLE IN NEUROLOGIC DISEASE [J].

EDDLESTON, M ;

MUCKE, L .

NEUROSCIENCE, 1993, 54 (01) :15-36

[6] DIFFERENTIATION OF T-CELL LYMPHOKINE GENE-EXPRESSION - THE INVITRO ACQUISITION OF T-CELL MEMORY [J].

EHLERS, S ;

SMITH, KA .

JOURNAL OF EXPERIMENTAL MEDICINE, 1991, 173 (01) :25-36

[7] FUNCTIONAL-CHARACTERIZATION OF NEUROKININ-1 RECEPTORS ON HUMAN U373MG ASTROCYTOMA-CELLS [J].

EISTETTER, HR ;

MILLS, A ;

BREWSTER, R ;

ALOUANI, S ;

RAMBOSSON, C ;

KAWASHIMA, E .

GLIA, 1992, 6 (02) :89-95

[8] PRODUCTION OF B-CELL STIMULATORY FACTOR-II AND INTERFERON-GAMMA IN THE CENTRAL NERVOUS-SYSTEM DURING VIRAL MENINGITIS AND ENCEPHALITIS - EVALUATION IN A MURINE MODEL INFECTION AND IN PATIENTS [J].

FREI, K ;

LEIST, TP ;

MEAGER, A ;

GALLO, P ;

LEPPERT, D ;

ZINKERNAGEL, RM ;

FONTANA, A .

JOURNAL OF EXPERIMENTAL MEDICINE, 1988, 168 (01) :449-453

[9] ON THE CELLULAR SOURCE AND FUNCTION OF INTERLEUKIN-6 PRODUCED IN THE CENTRAL NERVOUS-SYSTEM IN VIRAL DISEASES [J].

FREI, K ;

MALIPIERO, UV ;

LEIST, TP ;

ZINKERNAGEL, RM ;

SCHWAB, ME ;

FONTANA, A .

EUROPEAN JOURNAL OF IMMUNOLOGY, 1989, 19 (04) :689-694

[10] SOLUBLE CD14 PARTICIPATES IN THE RESPONSE OF CELLS TO LIPOPOLYSACCHARIDE [J].

FREY, EA ;

MILLER, DS ;

JAHR, TG ;

SUNDAN, A ;

BAZIL, V ;

ESPEVIK, T ;

FINLAY, BB ;

WRIGHT, SD .

JOURNAL OF EXPERIMENTAL MEDICINE, 1992, 176 (06) :1665-1671

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