CELL-WALL POLYSACCHARIDES OF 4 STRAINS OF PAECILOMYCES-VARIOTII

被引：6

作者：

DOMENECH, J

PRIETO, A

BERNABE, M

LEAL, JA

机构：

[1] CSIC,CTR INVEST BIOL,E-28006 MADRID,SPAIN

[2] INST ORGAN CHEM,CARBOHYDRATES SECT,MADRID,SPAIN

来源：

CURRENT MICROBIOLOGY | 1994年 / 28卷 / 03期

关键词：

D O I：

10.1007/BF01571060

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The main polysaccharidic fractions extracted with 1M NaOH from cell wall material of four different strains of Paecilomyces variotii were characterized as an alpha-(1 --> 3)-glucan (F1I) amounting from 33.2 to 39.1% and a beta-glucan-chitin complex (F4) representing from 42.7 to 47.3%. The water-soluble fraction, F1S, was composed of mannose, glucose, and mainly galactose in all the strains studied. The F1S fractions of the four strains were analyzed by NMR spectroscopy showing analogous structural features in all the strains. This fraction was purified through Sepharose CL-GB and methylated. The purified material (F1S-B) mainly contained (1 --> 6)- and (1 --> 2,6)-linked mannopyranose, (1 --> 5)- and (1 --> 6)-linked galactofuranose, and terminal residues of glucopyranose and galactofuranose. The proportion, the chemical composition, and the structure of each fraction revealed a homogeneous cell wall composition in these strains.

引用

页码：169 / 173

页数：5

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